Human sFRP-5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human sFRP-5 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 707128
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human sFRP-5
    Glu30-His317
    Accession # Q5T4F7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize sFRP-5 inhibited Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 2-10 μg/mL in the presence of 5 μg/mL Recombinant Human sFRP-5 and 100 ng/mL Recombinant Mouse Wnt-3a.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody. Recombinant Human sFRP‑5 (Catalog # 6266-SF) inhibits Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP‑5 (5 μg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP‑5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt‑3a. The ND50 is typically 2‑10 μg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: sFRP-5
Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP-2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down-regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial-mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down-regulated in mouse models of obesity and type 2 diabetes (10).
  • References:
    1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
    2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
    3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
    4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
    5. Leaf, I. et al. (2006) Genesis 44:573.
    6. Satoh, W. et al. (2008) Genesis 46:92.
    7. Li, Y. et al. (2008) Genes Dev. 22:3050.
    8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
    9. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
    10. Ouchi, N. et al. (2010) Science 329:454.
    11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
    12. Zhao, C. et al. (2009) BMC Cancer 9:224.
    13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
    14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.
  • Long Name:
    Secreted Frizzled Related Protein 5
  • Entrez Gene IDs:
    6425 (Human)
  • Alternate Names:
    frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP5; sFRP-5
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