Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 707128
View all sFRP-5 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human sFRP-5
Glu30-His317
Accession # Q5T4F7

Specificity

Detects human sFRP-5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human sFRP‑5 Antibody

Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody.

Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody.

Recombinant Human sFRP-5 (Catalog # 6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND50 is typically 2-10 µg/mL.

Applications for Human sFRP‑5 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize sFRP-5 inhibited Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 2-10 μg/mL in the presence of 5 μg/mL Recombinant Human sFRP-5 and 100 ng/mL Recombinant Mouse Wnt-3a.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-5

Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP-2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down-regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial-mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down-regulated in mouse models of obesity and type 2 diabetes (10).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Leaf, I. et al. (2006) Genesis 44:573.
  6. Satoh, W. et al. (2008) Genesis 46:92.
  7. Li, Y. et al. (2008) Genes Dev. 22:3050.
  8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
  9. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
  10. Ouchi, N. et al. (2010) Science 329:454.
  11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
  12. Zhao, C. et al. (2009) BMC Cancer 9:224.
  13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
  14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

Long Name

Secreted Frizzled Related Protein 5

Alternate Names

FRP1B, SARP3, sFRP5

Entrez Gene IDs

6425 (Human); 54612 (Mouse)

Gene Symbol

SFRP5

UniProt

Q5T4F7

Additional sFRP-5 Products

Product Documents for Human sFRP‑5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human sFRP‑5 Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

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