Human sFRP-5 Antibody (707128)

R&D Systems | Catalog # MAB6266

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 707128
View all sFRP-5 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human sFRP-5
Glu30-His317
Accession # Q5T4F7

Specificity

Detects human sFRP-5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human sFRP-5 Antibody (707128)

Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody.

Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody.

Recombinant Human sFRP-5 (Catalog # 6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND50 is typically 2-10 µg/mL.

Applications for Human sFRP-5 Antibody (707128)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize sFRP-5 inhibited Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 2-10 μg/mL in the presence of 5 μg/mL Recombinant Human sFRP-5 and 100 ng/mL Recombinant Mouse Wnt-3a.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-5

Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP-2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down-regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial-mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down-regulated in mouse models of obesity and type 2 diabetes (10).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Leaf, I. et al. (2006) Genesis 44:573.
  6. Satoh, W. et al. (2008) Genesis 46:92.
  7. Li, Y. et al. (2008) Genes Dev. 22:3050.
  8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
  9. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
  10. Ouchi, N. et al. (2010) Science 329:454.
  11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
  12. Zhao, C. et al. (2009) BMC Cancer 9:224.
  13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
  14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

Long Name

Secreted Frizzled Related Protein 5

Alternate Names

FRP1B, SARP3, sFRP5

Entrez Gene IDs

6425 (Human); 54612 (Mouse)

Gene Symbol

SFRP5

UniProt

Q5T4F7

Additional sFRP-5 Products

Product Documents for Human sFRP-5 Antibody (707128)

Certificate of Analysis

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Product Specific Notices for Human sFRP-5 Antibody (707128)

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

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