Detects human SIGIRR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) IL‑1 RI, rhIL‑1 R3, rhIL-1 R8, rhIL-1 RII, rhIL-1 R6, rhIL-1 R4, rhIL-18, rhIL-1 R7, rhIL-9 R9, or recombinant mouse SIGIRR.
Monoclonal Mouse IgG2A Clone # 162201
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human SIGIRR Met1-His118 Accession # Q6IA17
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SIGIRR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) were stained with Mouse Anti-Human SIGIRR APC‑conjugated Monoclonal Antibody (Catalog # FAB990A) and Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 R beta (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Five of the IL-1 R family members (1, 2, 4, 5, and 6) are clustered and localized to chromosome 2. SIGIRR (single Ig domain containing IL-1 receptor-related molecule) is a subtype of the IL-1 R family that differs from the other nine members by having only one Ig domain in its extracellular region. The sequence of human SIGIRR predicts a 410 amino acid (aa) residue transmembrane glycoprotein that lacks signal peptide and contains a 118 aa single Ig extracellular domain, a transmembrane region and a 268 aa cytoplasmic tail with a TIR domain. The cytoplasmic tail of SIGIRR contains a C-terminal extention beyond the TIR domain which is also found in IL-1 R8, IL-1 R9, and Toll-like receptor family members but absent in other IL-1 receptor family members. SIGIRR is widely expressed and is present in all cells and tissues examined. Mouse and human SIGIRR share 82% amino acid sequence identity. The ligand and signaling mechanism for SIGIRR has not been identified.
Thomassen, E. et al. (1999) Cytokine 11:389.
Single lg IL-1 Related Protein
Entrez Gene IDs:
59307 (Human); 24058 (Mouse)
single Ig IL-1-related receptor; Single Ig IL-1R-related molecule; single immunoglobulin and toll-interleukin 1 receptor (TIR) domain; single immunoglobulin domain IL1R1 related; Single immunoglobulin domain-containing IL1R-related protein; TIR8; TIR8MGC110992; Toll/interleukin-1 receptor 8
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