Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of SLC5A8/SMCT1 in HEK293 Human Cell Line Transfected with Human SLC5A8/SMCT1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 and eGFP was stained with either (A) Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SLC5A8/SMCT1 (Solute Carrier family 5, member 8) is a member of the solute carrier family of multi-pass membrane proteins. SLC5A8 has been shown to transport iodide by a passive mechanism. It also transports monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism. SLC5A8 nuclear translocation and loss of expression are associated with poor outcome in pancreatic ductal adenocarcinoma (1). It may be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract.
Helm, J. et al. (2012) Pancreas 41:904.
Solute Carrier Family 5 Iodide Transporter), Member 8
Entrez Gene IDs:
160728 (Human); 216225 (Mouse); 500820 (Rat)
AIT; AITSolute carrier family 5 member 8; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; MGC125354; SLC5A8; SMCT; SMCTSMCT1; Sodium iodide-related cotransporter; sodium-coupled monocarboxylate transporter 1; solute carrier family 5 (iodide transporter), member 8
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