Human SLITRK6 Antibody

Catalog # Availability Size / Price Qty
AF7317
AF7317-SP
Detection of Human SLITRK6 by Western Blot.
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Human SLITRK6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLITRK6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SLITRK2, rhSLITRK4, and rhSLITRK6 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SLITRK6
Ile16-Ala604
Accession # Q9H5Y7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SLITRK6 by Western Blot. View Larger

Detection of Human SLITRK6 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human SLITRK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7317) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLITRK6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK6

SLITRK6 (Slit and NTRK-like 6) is a 95 kDa (predicted) member of the SLITRK family of proteins. It is widely expressed in the embryo, including cells in limb bud mesenchyme, visceral organ primodia, cells of the dorsal thalamus, and cochlear plus vestibular sensory epithelium. In the ear, SLITRK6 is suggested to induce neurotrophin expression from cells, thus promoting sensory neuron survival and axon pathfinding. Mature human SLITRK6 is an 826 amino acid (aa) type I transmembrane protein. It contains a 593 aa extracellular domain (ECD) (aa 16-608) plus a 212 aa cytoplasmic region. The ECD possesses two LRR clusters, one that involves seven LRRs (aa 27-269), and a second that involves eight LRRs (aa 320-568). Over aa 16-604, human SLITRK6 shares 89% aa sequence identity with mouse SLITRK6.

Long Name
SLIT and NTRK-like Family, Member 6
Entrez Gene IDs
84189 (Human); 239250 (Mouse); 290467 (Rat)
Alternate Names
FLJ22774; MGC119595; MGC119596; MGC119597,4832410J21Rik; SLIT and NTRK-like family, member 6; SLIT and NTRK-like protein 6; slit and trk like gene 6; SLITRK6

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