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Human SLPI Antibody

Catalog #: AB-260-NA
2 Citations Datasheet / COA / SDS
Newer Version Available: AF1274
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Discontinued Product

AB-260-NA has been discontinued and is replaced by AF1274.

SLPI in Human Lung.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLPI Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse SLPI is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human SLPI
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL

Recombinant Human SLPI, under non-reducing conditions only

Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry SLPI antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

SLPI in Human Lung. SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Polyclonal Antibody (Catalog # AB‑260‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLPI

Secretory Leukocyte Protease Inhibitor (SLPI) is a protein found in various secretory fluids, including parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid. The protein is a potent inhibitor of a variety of proteolytic enzymes, including the neutrophil proteases, elastase and cathepsin G and the pancreatic proteases chymotrypsin and trypsin. SLPI is an important component of the antiprotease defense of tissues bathed by secretory fluids. It could be used as a therapeutic agent for treatment of the proteolytic damage of tissues seen in degenerative and inflammatory diseases.

Long Name
Secretory Leukocyte Protease Inhibitor
Entrez Gene IDs
6590 (Human); 20568 (Mouse)
Alternate Names
ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI

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Citations for Human SLPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions
    Authors: Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al.
    Journal of Immunological Methods
    Species: Human
    Sample Types: Vaginal Lavage Fluid
    Applications: ELISA Detection
  2. Streptococcal DRS (distantly related to SIC) and SIC inhibit antimicrobial peptides, components of mucosal innate immunity: a comparison of their activities.
    Authors: Fernie-King BA, Seilly DJ, Binks MJ, Sriprakash KS, Lachmann PJ
    Microbes Infect., 2007-01-08;9(3):300-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization

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