Human SLPI Quantikine ELISA Kit

R&D Systems | Catalog # DPI00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL), Urine (20 µL)

Sensitivity

25 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
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Product Summary for Human SLPI Quantikine ELISA Kit

The Quantikine Human SLPI Immunoassay is a 4.5 hour solid phase ELISA designed to measure SLPI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human SLPI and antibodies raised against the recombinant factor. The kit has been shown to accurately quantitate recombinant human SLPI. Results obtained using natural human SLPI showed linear curves that were parallel to the curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SLPI.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human SLPI

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 109 693 2026 188 1174 2157
Standard Deviation 8.7 37.1 84.1 15.1 57.5 123
CV% 8.0 5.4 4.2 8.0 4.9 5.7

Recovery for Human SLPI Quantikine ELISA Kit

The recovery of SLPI was determined in various matrices by mixing high dose samples with low dose samples in ratios of 1:2, 1:1, and 2:1.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 94 84-105
Citrate Plasma (n=10) 97 92-102
EDTA Plasma (n=10) 96 87-103
Heparin Plasma (n=10) 106 98-116
Serum (n=10) 98 89-104
Urine (n=25) 98 92-103

Linearity

To assess the linearity of the assay, the following biological samples containing, or spiked with high concentrations of SLPI, were diluted with Calibrator Diluent and then assayed.

Human SLPI ELISA Linearity

Scientific Data Images for Human SLPI Quantikine ELISA Kit

Human SLPI ELISA Standard Curve

Human SLPI ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SLPI

Secretory leukocyte protease inhibitor (SLPI) is found in various secretory fluids including parotid secretions, bronchial, nasal, cervical mucous, and seminal fluid. SLPI is a potent inhibitor of many proteolytic enzymes including the neutrophil proteases, elastase, cathepsin G, chymotrypsin, and trypsin.

Long Name

Secretory Leukocyte Protease Inhibitor

Alternate Names

ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI

Entrez Gene IDs

6590 (Human); 20568 (Mouse)

Gene Symbol

SLPI

Additional SLPI Products

Product Documents for Human SLPI Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SLPI Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human SLPI Quantikine ELISA Kit

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Protocols

View specific protocols for Human SLPI Quantikine ELISA Kit (DPI00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 3 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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