Human Soluble Receptor Array Kit Hematopoietic Panel


R&D Systems Human Soluble Receptor Array Kit Hematopoietic Panel (Catalog # ARY011) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 105 different soluble receptors expressed and released by hematopoietic cells in a single sample. No specialized equipment is necessary.

Sample Data

Simultaneously detect the levels of these soluble receptors in a single sample.
Hematopoeitic Array     Common Analytes Array
CD5 Integrin alpha 5/CD49e     ACE HB-EGF
CD6 Integrin alpha 6/CD49f     ADAM8 ICAM-2/CD102
CD30 Integrin alpha 9     ADAM9 IL-1 RII
CD40 Integrin alpha E/CD103     ADAM10 IL-15 R alpha
CD43 Integrin alpha L/CD11a     ALCAM/CD166 Integrin beta 1/CD29
CD48/SLAMF2 Integrin alpha M/CD11b     Amphiregulin Integrin beta 2/CD18
CD59 Integrin alpha X/CD11c     APP (pan) Integrin beta 3/CD61
CD84/SLAMF5 LAG-3     BACE-1 Integrin beta 4/CD104
CD97 MMP-9 (total)     BCAM/CD239 Integrin beta 5
CD163 MMR     C1q R1/CD93 Integrin beta 6
CD229/SLAMF3 Myeloperoxidase     CD9 JAM-A/CD321
Chitinase 3-like 1/YKL40 Resistin     CD23/Fc epsilon RII Lipocalin-2/NGAL
CRP L-Selectin     CD31/PECAM-1 LOX-1/SR-E1/CLEC8A
CRTAM Siglec-5     CD36/SR-B3 MD-1/LY86
CXCL16 Siglec-6     CD40 Ligand MMP-2 (total)
DNAM-1 Siglec-7     CD44H NCAM-1/CD56
DPPIV/CD26 Siglec-9     CD58/LFA-3 NCAM-L1/CD171
IFN-gamma R2 Siglec-10     CD90/Thy1 Osteopontin
IL-2 R alpha TIM-3/KIM-3     CD99 PAR1
IL-2 R beta TLR2/CD282     CD155/PVR Pref-1/DLK-1/FA1
IL-6 R TLR4/CD284     CEACAM-1/CD66a RECK
Integrin alpha 3/CD49c TNF RI     CX3CL1/Fractalkine Stabilin-1
Integrin alpha 4/CD49d TRACP/PAP/ACP5     CXCL8/IL-8 TACE/ADAM17
  TRANCE/RANKL     EMMPRIN/CD147 Thrombospondin-1
  TREM-1     Endoglin/CD105 TIMP-1
        Epiregulin TIMP-2
        Galectin-1 TIMP-3
        Galectin-3 TNF RII

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

  • 2 Hematopoeitic Array Membranes
  • 2 Common Analyte Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided and Other Materials Required sections of the product datasheet.