Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
R&D Systems | Catalog # DSHH00
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Product Specifications
Measurement
Detection Method
Conjugate
Species
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 99.5 | 291 | 532 | 109 | 318 | 612 |
| Standard Deviation | 5.97 | 8.15 | 14.4 | 10.3 | 24.5 | 50.3 |
| CV% | 6.0 | 2.8 | 2.7 | 9.4 | 7.7 | 8.2 |
Recovery for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
The recovery of human Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 108 | 100-117 |
| Heparin Plasma (n=4) | 97 | 85-117 |
| Serum (n=4) | 99 | 81-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Human Sonic Hedgehog/Shh N-Terminus ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Sonic Hedgehog/Shh
Alternate Names
Gene Symbol
Additional Sonic Hedgehog/Shh Products
Product Documents for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
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FAQs for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
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Q: Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
