Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

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Human Sonic Hedgehog/Shh N-Terminus Standard Curve
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Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (34 uL), Serum (34 uL), Heparin Plasma (34 uL)
Sensitivity
3.92 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Sonic Hedgehog/Shh N-Terminus Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Shh-N in cell culture supernates, serum, and heparin plasma. It contains E. coli-expressed recombinant human Shh-N and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Shh-N showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Shh-N.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components.<br>

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 99.5 291 532 109 318 612
Standard Deviation 5.97 8.15 14.4 10.3 24.5 50.3
CV% 6 2.8 2.7 9.4 7.7 8.2

Recovery

The recovery of human Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 100-117
Heparin Plasma (n=4) 97 85-117
Serum (n=4) 99 81-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human Sonic Hedgehog/Shh N-Terminus ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Sonic Hedgehog/Shh

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

Entrez Gene IDs:
6469 (Human); 20423 (Mouse)
Alternate Names:
HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Hedgehog signaling contributes to bone cancer pain by regulating sensory neuron excitability in rats
    Authors: S Liu, Y Lv, XX Wan, ZJ Song, YP Liu, S Miao, GL Wang, GJ Liu
    Mol Pain, 2018;14(0):1744806918767.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia
    Authors: C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu
    Mucosal Immunol, 2018;0(0):.
    Species: Human
    Sample Types: BALF

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