Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (34 uL), Serum (34 uL), Heparin Plasma (34 uL)
  • Sensitivity
    3.92 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC219, Quantikine Immunoassay Control Set 882 for Human Shh-N - Please Inquire
Product Summary
The Quantikine Human Sonic Hedgehog/Shh N-Terminus Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Shh-N in cell culture supernates, serum, and heparin plasma. It contains E. coli-expressed recombinant human Shh-N and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Shh-N showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Shh-N.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean99.5291532109318612
Standard Deviation5.978.1514.410.324.550.3
CV%62.82.79.47.78.2

Recovery

The recovery of human Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 100-117
Heparin Plasma (n=4) 97 85-117
Serum (n=4) 99 81-117
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Sonic Hedgehog/Shh
Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
    • Entrez Gene IDs
      6469 (Human); 20423 (Mouse);
    • Alternate Names
      HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS;
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 882 for Human Shh-N

    Ctrl QC219
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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