Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SorCS1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SorCS1
Ser111-Ser1099 (Ser231Gly)
Accession # Q8WY21.3

Specificity

Detects human SorCS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse SorCS1 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS2 and rhSorCS3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SorCS1 Antibody

Detection of SorCS1 by Western Blot

Detection of SorCS1 by Western Blot

MPEP decreases SORT1 expression and increases extracellular PGRN in mammalian cell lines. (A and B) M17 cells were treated with control siRNA (siR-Ctrl) or gene-specific SORT1 siRNA (siR-SORT1). (A) Intracellular levels of PGRN, SORT1 and GAPDH were evaluated by western blot at a 48 h time-point. (B) Suppression of SORT1 levels increased extracellular PGRN levels. (C) Chemical name and structure of MPEP. (D–I) Treatment of M17 cells (D and E), HeLa cells (F and G) or NIH3T3 cells (H and I) with MPEP for 24 h dose dependently reduced SORT1 levels (D, F and H) and increased exPGRN levels (E, G and I) at 10 and 20 μM. (J) Under the same conditions, MPEP did not affect levels of SORLA, SORCS1 and ubiquitinated proteins in M17 cells. ***P < 0.001 versus vehicle control, analysis performed by one-way ANOVA followed by Tukey's post-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24163244), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human SorCS1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human SorCS1 (Catalog # 3457-SR)

Reviewed Applications

Read 3 reviews rated 5 using AF3457 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SorCS1

SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2‑5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).

References

  1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
  3. Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.
  4. Clee, S.M. et al. (2006) Nat. Genet. 38:688.
  5. Hermey, G. et al. (2001) Neurosci. Lett. 313:83.
  6. Hermey, G. et al. (2006) Biochem. J. 395:285.
  7. Goodarzi, M.O. et al. (2007) Diabetes Apr 10 [Epub ahead of print].
  8. Nyborg, A.C. et al. (2006) Mol. Neurodegen. 1:3.

Long Name

Sortilin-related VPS10 Domain Containing Receptor 1

Alternate Names

FLJ41758, FLJ43475, FLJ44957, hSorCS, SORCS, SORCS receptor 1, sorCS1, sortilin-related VPS10 domain containing receptor 1, VPS10 domain receptor protein SORCS 1, VPS10 domain receptor SorCS, VPS10 domain-containing receptor SorCS1

Entrez Gene IDs

114815 (Human); 58178 (Mouse); 309533 (Rat)

Gene Symbol

SORCS1

UniProt

Q8WY21.3

Additional SorCS1 Products

Product Documents for Human SorCS1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SorCS1 Antibody

For research use only

Related Research Areas

Diabetes

Citations for Human SorCS1 Antibody

Customer Reviews for Human SorCS1 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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2 Stars
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1 Stars
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Customer Images

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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: IP of human SorCS1 transfected into HEK293 cells
    Species: Human
    Verified Customer | Posted 12/18/2014
    Human SorCS1 Antibody AF3457
  • Name: Anonymous
    Application: ELISA
    Sample Tested: N/A
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: CHO-cells stable expressing mouse SorCS1 variant c
    Species: Other
    Verified Customer | Posted 12/18/2014

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