Human SorCS1 Antibody

Catalog # Availability Size / Price Qty
AF3457
AF3457-SP
Product Details
Citations (2)
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Human SorCS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SorCS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse SorCS1 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS2 and rhSorCS3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SorCS1
Ser111-Ser1099 (Ser231Gly)
Accession # Q8WY21.3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SorCS1 (Catalog # 3457-SR)
Immunocytochemistry
5-15 µg/mL
Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorCS1

SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2‑5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).

References
  1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
  3. Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.
  4. Clee, S.M. et al. (2006) Nat. Genet. 38:688.
  5. Hermey, G. et al. (2001) Neurosci. Lett. 313:83.
  6. Hermey, G. et al. (2006) Biochem. J. 395:285.
  7. Goodarzi, M.O. et al. (2007) Diabetes Apr 10 [Epub ahead of print].
  8. Nyborg, A.C. et al. (2006) Mol. Neurodegen. 1:3.
Long Name
Sortilin-related VPS10 Domain Containing Receptor 1
Entrez Gene IDs
114815 (Human); 58178 (Mouse); 309533 (Rat)
Alternate Names
FLJ41758; FLJ43475; FLJ44957; hSorCS; SORCS receptor 1; SORCS; SorCS1; sortilin-related VPS10 domain containing receptor 1; VPS10 domain receptor protein SORCS 1; VPS10 domain receptor SorCS; VPS10 domain-containing receptor SorCS1

Product Datasheets

Citations for Human SorCS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017;13(6):e1006470.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. SORCS1 alters amyloid precursor protein processing and variants may increase Alzheimer's disease risk.
    Authors: Reitz C, Tokuhiro S, Clark LN, Conrad C, Vonsattel JP, Hazrati LN, Palotas A, Lantigua R, Medrano M, Z Jimenez-Velazquez I, Vardarajan B, Simkin I, Haines JL, Pericak-Vance MA, Farrer LA, Lee JH, Rogaeva E, George-Hyslop PS, Mayeux R
    Ann. Neurol., 2011;69(1):47-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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