Human Sphingosine Kinase 1/SPHK1 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # AF5536AFP405

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry, Immunoprecipitation

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Sheep IgG
View all Sphingosine Kinase 1/SPHK1 Primary Antibodies »

Product Specifications

Specificity

Detects human SPHK1 in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human SPHK2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

ELISA

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Sphingosine Kinase 1/SPHK1

Sphingosine kinases are cytosolic or membrane-associated enzymes that catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N‑terminal and central regions (1). The two proteins also differ in tissue distribution and some kinetic properties (1). S1P is a lipid messenger that regulates diverse physiological processes including cell proliferation, migration, apoptosis, inflammation, calcium homeostasis and cytoskeletal structure (2, 3). The level of S1P is tightly controlled by SPHKs and S1P degrading enzymes. SPHK1 and its activation can be stimulated by several growth factors such as tumor necrosis factor-alpha, epidermal growth factor and transforming growth factor-beta (3, 4). Expression of SPHK1 has been found to increase in many human solid tumors and overexpression of SPHK1 is associated with tumor angiogenesis (5). Such studies have implicated SPHK1 as a new target for cancer treatment.

References

  1. Liu, H. et al. (2000) J. Biol. Chem. 275:19513.
  2. Spiegel, S. (1999) J. Leukocyte Biol. 65:341.
  3. Alemany, R. et al. (2007) Naunyn-Schmiedegerg’s Arch. Pharmacol. 374:413.
  4. Pederson, L. et al. (2008) Proc. Natl. Acad. Scis USA. 105:20764.
  5. Shida, D. et al. (2008) Curr. Drug Targets. 9:662.

Alternate Names

SK1, SPK1

Entrez Gene IDs

8877 (Human); 20698 (Mouse); 170897 (Rat)

Gene Symbol

SPHK1

UniProt

NP_068807

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways
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