Detection of Human SPRED1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human SPRED1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5067) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED1 at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6‑123), a central KBD domain that binds to the SCFR (aa 233‑285), and a C-terminal Cys‑rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334‑442). There is one splice variant that shows a nine Lys substitution for aa 269‑444.
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