Human SPRED1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SPRED1 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human SPRED1 Antibody Summary

Species Reactivity
Detects endogenous human SPRED1 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human SPRED1
Accession # Q7Z699
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SPRED1 antibody by Western Blot. View Larger

Detection of Human SPRED1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human SPRED1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED1 at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPRED1

SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6‑123), a central KBD domain that binds to the SCFR (aa 233‑285), and a C-terminal Cys‑rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334‑442). There is one splice variant that shows a nine Lys substitution for aa 269‑444.

Long Name
Sprouty-related with an EVH1 Domain Containing-1
Entrez Gene IDs
161742 (Human); 114715 (Mouse); 296072 (Rat)
Alternate Names
EVH1/Sprouty domain containing protein; FLJ33903; hSpred1; NFLS; SPRED1; spred-1; sprouty-related, EVH1 domain containing 1; sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation

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Citation for Human SPRED1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A shared molecular mechanism underlies the human rasopathies Legius syndrome and Neurofibromatosis-1.
    Authors: Stowe IB, Mercado EL, Stowe TR, Bell EL, Oses-Prieto JA, Hernandez H, Burlingame AL, McCormick F
    Genes Dev., 2012;26(13):1421-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human SPRED1 Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human SPRED1 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human