Human SPRY3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human SPRY3 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SPRY3
    Met1-Glu142
    Accession # O43610
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SPRY3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5735) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPRY3
SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288 amino acids (aa) in length and contains one SH2-binding domain (aa 25‑30), a PEST sequence (aa 82‑93), and a Cys-rich domain (aa 147‑270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes.
  • Long Name:
    Sprouty Homolog 3
  • Entrez Gene IDs:
    10251 (Human); 236576 (Mouse)
  • Alternate Names:
    Antagonist Of FGF Signaling; HSPRY3; Protein Sprouty Homolog 3; sprouty (Drosophila) homolog 2; Sprouty Homolog 3 (Drosophila); SPRY3; spry-3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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