Human ST2/IL-33 R Quantikine ELISA Kit

(11 citations)
(2 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    13.5 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human ST2 as well as free ST2 and IL-33 complexed ST2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC162, Quantikine Immunoassay Control Set 873 for Human ST2/IL-33 R - Please Inquire
Product Summary
The Quantikine Human ST2/IL-33 R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ST2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human ST2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ST2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ST2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean27362810272626421064
Standard Deviation15.227.546.318.734.767.2
CV%5.64.44.57.15.46.3

Recovery

The recovery of ST2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 89-106
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ST2/IL-33 R Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ST2/IL-33 R
ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
    • Long Name
      Interleukin 33 Receptor
    • Entrez Gene IDs
      9173 (Human); 17082 (Mouse);
    • Alternate Names
      DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1;
    Related Research Areas
    Assay Procedure
    Refer to the product datasheet for complete assay procedure.
     
    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

    Prepare all reagents, standard dilutions, and samples as directed in the product insert.

    Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    Add 50 µL of Assay Diluent to each well.

    add diluent to well graphic

    Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

    add diluent to well graphic

    Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

    add diluent to well graphic

    Aspirate and wash 4 times.

    Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    add substrate to well graphic

    Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    add stop solution to well graphic
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    11 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
      Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
      Hellenic J Cardiol, 2017;0(0):.
      Species: Human
      Sample Type: Serum
    2. The injured heart: early cardiac effects of hematopoietic stem cell transplantation in children and young adults
      Authors: SJ Rotz, TD Ryan, S Jodele, JL Jefferies, A Lane, A Pate, R Hirsch, J Hlavaty, AE Levesque, MD Taylor, M Cash, KC Myers, JA El-Bietar, SM Davies, CE Dandoy
      Bone Marrow Transplant., 2017;0(0):.
      Species: Human
      Sample Type: Plasma
    3. Long-term systolic function in children and young adults after hematopoietic stem cell transplant
      Authors: SJ Rotz, CE Dandoy, MD Taylor, S Jodele, JL Jefferies, A Lane, JA El-Bietar, AW Powell, SM Davies, TD Ryan
      Bone Marrow Transplant., 2017;0(0):.
      Species: Human
      Sample Type: Plasma
    4. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
      Nat Commun, 2016;7(0):13710.
      Species: Human
      Sample Type: Plasma
    5. IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline
      Authors: AK Fu, KW Hung, MY Yuen, X Zhou, DS Mak, IC Chan, TH Cheung, B Zhang, WY Fu, FY Liew, NY Ip
      Proc Natl Acad Sci USA, 2016;0(0):.
      Species: Human
      Sample Type: Serum
    6. Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
      Sci Rep, 2016;6(0):32116.
      Species: Human
      Sample Type: Plasma
    7. Phenotypic and genotypic association of epithelial IL1RL1 to human TH2-like asthma.
      Authors: Traister R, Uvalle C, Hawkins G, Meyers D, Bleecker E, Wenzel S
      J Allergy Clin Immunol, 2015;135(1):92-9.
      Species: Human
      Sample Type: BALF
    8. Reduced IL-33 plasma levels in aplastic anemia.
      Authors: Sun M, Ma H, Che Y, Cui X
      Cancer Cell Int, 2015;15(0):118.
      Species: Human
      Sample Type: Plasma
    9. IL1RL1 gene variants and nasopharyngeal IL1RL-a levels are associated with severe RSV bronchiolitis: a multicenter cohort study.
      Authors: Faber TE, Schuurhof A, Vonk A, Koppelman GH, Hennus MP, Kimpen JL, Janssen R, Bont LJ
      PLoS ONE, 2012;7(5):e34364.
      Species: Human
      Sample Type: Tissue Secretion
    10. High Levels of Soluble ST2 and Low Levels of IL-33 in Sera of Patients with HIV Infection.
      Authors: Miyagaki T, Sugaya M
      J. Invest. Dermatol., 2010;131(0):794.
      Species: Human
      Sample Type: Serum
    11. IL-33 mediates inflammatory responses in human lung tissue cells.
      Authors: Yagami A, Orihara K, Morita H
      J. Immunol., 2010;185(10):5743-50.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 11 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 873 for Human ST2/IL-33 R

    Ctrl QC162
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 4 (Based on 2 reviews)

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    Images Ratings Applications Species Reviewed By Date Details
     Human ST2/IL-33 R Quantikine ELISA Kit DST200
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    Very Good
      Anonymous 12/01/2016
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     Human ST2/IL-33 R Quantikine ELISA Kit DST200
    : Human ST2/IL-33 R Quantikine ELISA Kit [DST200]

    Summary

    Sample TestedHuman serum
      Very Good
      Anonymous 07/26/2016
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    Summary

    Sample TestedEDTA Plasma,Cell culture supernatant,Cell Lysates

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