Human ST2/IL-33R Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 273 | 628 | 1027 | 262 | 642 | 1064 |
Standard Deviation | 15.2 | 27.5 | 46.3 | 18.7 | 34.7 | 67.2 |
CV% | 5.6 | 4.4 | 4.5 | 7.1 | 5.4 | 6.3 |
Recovery
The recovery of ST2 spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 97 | 89-106 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: ST2/IL-33R
ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
Assay Procedure
Refer to the product datasheet for complete assay procedure.Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
Add 50 µL of Assay Diluent to each well.

Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

Aspirate and wash 4 times.
Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human ST2/IL-33R Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
21
Citations: Showing 1 - 10
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Immune Profiling To Predict Outcome of Clostridioides difficile Infection
Authors: MM Abhyankar, JZ Ma, KW Scully, AJ Nafziger, AL Frisbee, MM Saleh, GR Madden, AR Hays, M Poulter, WA Petri
MBio, 2020;11(3):.
Species: Human
Sample Types:
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Systemic Inflammation Precedes Microalbuminuria in Diabetes
Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk,
Kidney Int Rep, 2019;4(10):1373-1386.
Species: Human
Sample Types: Serum
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Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
Cytokine, 2018;0(0):.
Species: Human
Sample Types: Plasma
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The prognostic value of sST2 and galectin-3 considering different aetiologies in non-ischaemic heart failure
Authors: D Binas, H Daniel, A Richter, V Ruppert, KD Schlüter, B Schieffer, S Pankuweit
Open Heart, 2018;5(1):e000750.
Species: Human
Sample Types: Serum
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Genetic regulation ofIL1RL1methylation and IL1RL1-a protein levels in asthma
Authors: FN Dijk, C Xu, E Melén, AE Carsin, A Kumar, IM Nolte, O Gruzieva, G Pershagen, NS Grotenboer, OEM Savenije, JM Antó, I Lavi, C Dobaño, J Bousquet, P van der Vl, RJP van der Va, JC de Jongste, MC Nawijn, S Guerra, DS Postma, GH Koppelman
Eur. Respir. J., 2018;51(3):.
Species: Human
Sample Types: Serum
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Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
J Clin Med, 2018;7(11):.
Species: Human
Sample Types: Plasma
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Biomarkers as predictors of sudden cardiac death in coronary artery disease patients with preserved left ventricular function (ARTEMIS study)
Authors: ES Lepojärvi, HV Huikuri, OP Piira, AM Kiviniemi, JA Miettinen, T Kenttä, O Ukkola, JS Perkiömäki, MP Tulppo, MJ Junttila
PLoS ONE, 2018;13(9):e0203363.
Species: Human
Sample Types: Serum
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Serum sST2 levels predict severe exacerbation of asthma
Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
Respir. Res., 2018;19(1):169.
Species: Human
Sample Types: Serum
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Interleukin-33 modulates inflammation in endometriosis
Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
Sci Rep, 2017;7(1):17903.
Species: Human
Sample Types: Plasma
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Long-term systolic function in children and young adults after hematopoietic stem cell transplant
Authors: SJ Rotz, CE Dandoy, MD Taylor, S Jodele, JL Jefferies, A Lane, JA El-Bietar, AW Powell, SM Davies, TD Ryan
Bone Marrow Transplant., 2017;0(0):.
Species: Human
Sample Types: Plasma
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Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
Hellenic J Cardiol, 2017;0(0):.
Species: Human
Sample Types: Serum
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The injured heart: early cardiac effects of hematopoietic stem cell transplantation in children and young adults
Authors: SJ Rotz, TD Ryan, S Jodele, JL Jefferies, A Lane, A Pate, R Hirsch, J Hlavaty, AE Levesque, MD Taylor, M Cash, KC Myers, JA El-Bietar, SM Davies, CE Dandoy
Bone Marrow Transplant., 2017;0(0):.
Species: Human
Sample Types: Plasma
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Plasma Level of Soluble ST2 in Chronically Infected HIV-1 Patients with Suppressed Viremia
Authors: M Younas, C Psomas, V Mehraj, R Cezar, P Portales, E Tuaillon, A Guigues, J Reynes, P Corbeau, JP Routy
Open AIDS J, 2017;11(0):32-35.
Species: Human
Sample Types: Plasma
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IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline
Authors: AK Fu, KW Hung, MY Yuen, X Zhou, DS Mak, IC Chan, TH Cheung, B Zhang, WY Fu, FY Liew, NY Ip
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Types: Serum
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Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
Nat Commun, 2016;7(0):13710.
Species: Human
Sample Types: Plasma
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Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
Sci Rep, 2016;6(0):32116.
Species: Human
Sample Types: Plasma
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Phenotypic and genotypic association of epithelial IL1RL1 to human TH2-like asthma.
Authors: Traister R, Uvalle C, Hawkins G, Meyers D, Bleecker E, Wenzel S
J Allergy Clin Immunol, 2015;135(1):92-9.
Species: Human
Sample Types: BALF
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Reduced IL-33 plasma levels in aplastic anemia.
Authors: Sun M, Ma H, Che Y, Cui X
Cancer Cell Int, 2015;15(0):118.
Species: Human
Sample Types: Plasma
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IL1RL1 gene variants and nasopharyngeal IL1RL-a levels are associated with severe RSV bronchiolitis: a multicenter cohort study.
Authors: Faber TE, Schuurhof A, Vonk A, Koppelman GH, Hennus MP, Kimpen JL, Janssen R, Bont LJ
PLoS ONE, 2012;7(5):e34364.
Species: Human
Sample Types: Tissue Secretion
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IL-33 mediates inflammatory responses in human lung tissue cells.
Authors: Yagami A, Orihara K, Morita H
J. Immunol., 2010;185(10):5743-50.
Species: Human
Sample Types: Cell Culture Supernates
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High Levels of Soluble ST2 and Low Levels of IL-33 in Sera of Patients with HIV Infection.
Authors: Miyagaki T, Sugaya M
J. Invest. Dermatol., 2010;131(0):794.
Species: Human
Sample Types: Serum
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