Human ST2/IL-33R Quantikine ELISA Kit

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DST200
Control Products Available
Human ST2/IL-1 R4 ELISA Standard Curve
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Product Details
Procedure
Citations (17)
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Human ST2/IL-33R Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
13.5 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ST2 as well as free ST2 and IL-33 complexed ST2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ST2/IL-33 R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ST2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human ST2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ST2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ST2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 273 628 1027 262 642 1064
Standard Deviation 15.2 27.5 46.3 18.7 34.7 67.2
CV% 5.6 4.4 4.5 7.1 5.4 6.3

Recovery

The recovery of ST2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 89-106

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ST2/IL-1 R4 ELISA Linearity

Data Examples

Human ST2/IL-1 R4 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ST2/IL-33R

ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

Long Name:
Interleukin 33 Receptor
Entrez Gene IDs:
9173 (Human); 17082 (Mouse)
Alternate Names:
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product datasheet for complete assay procedure.
 
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.

Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

Add 50 µL of Assay Diluent to each well.

add diluent to well graphic

Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

add diluent to well graphic

Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

add diluent to well graphic

Aspirate and wash 4 times.

Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

add substrate to well graphic

Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

add stop solution to well graphic

Citations for Human ST2/IL-33R Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018;7(11):.
    Species: Human
    Sample Types: Plasma
  2. Biomarkers as predictors of sudden cardiac death in coronary artery disease patients with preserved left ventricular function (ARTEMIS study)
    Authors: ES Lepojärvi, HV Huikuri, OP Piira, AM Kiviniemi, JA Miettinen, T Kenttä, O Ukkola, JS Perkiömäki, MP Tulppo, MJ Junttila
    PLoS ONE, 2018;13(9):e0203363.
    Species: Human
    Sample Types: Serum
  3. Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
    Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
    Cytokine, 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  4. Serum sST2 levels predict severe exacerbation of asthma
    Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
    Respir. Res., 2018;19(1):169.
    Species: Human
    Sample Types: Serum
  5. The injured heart: early cardiac effects of hematopoietic stem cell transplantation in children and young adults
    Authors: SJ Rotz, TD Ryan, S Jodele, JL Jefferies, A Lane, A Pate, R Hirsch, J Hlavaty, AE Levesque, MD Taylor, M Cash, KC Myers, JA El-Bietar, SM Davies, CE Dandoy
    Bone Marrow Transplant., 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Interleukin-33 modulates inflammation in endometriosis
    Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
    Sci Rep, 2017;7(1):17903.
    Species: Human
    Sample Types: Plasma
  7. Long-term systolic function in children and young adults after hematopoietic stem cell transplant
    Authors: SJ Rotz, CE Dandoy, MD Taylor, S Jodele, JL Jefferies, A Lane, JA El-Bietar, AW Powell, SM Davies, TD Ryan
    Bone Marrow Transplant., 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Plasma Level of Soluble ST2 in Chronically Infected HIV-1 Patients with Suppressed Viremia
    Authors: M Younas, C Psomas, V Mehraj, R Cezar, P Portales, E Tuaillon, A Guigues, J Reynes, P Corbeau, JP Routy
    Open AIDS J, 2017;11(0):32-35.
    Species: Human
    Sample Types: Plasma
  9. Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
    Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
    Hellenic J Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  10. Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
    Sci Rep, 2016;6(0):32116.
    Species: Human
    Sample Types: Plasma
  11. IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline
    Authors: AK Fu, KW Hung, MY Yuen, X Zhou, DS Mak, IC Chan, TH Cheung, B Zhang, WY Fu, FY Liew, NY Ip
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Types: Serum
  12. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
    Nat Commun, 2016;7(0):13710.
    Species: Human
    Sample Types: Plasma
  13. Phenotypic and genotypic association of epithelial IL1RL1 to human TH2-like asthma.
    Authors: Traister R, Uvalle C, Hawkins G, Meyers D, Bleecker E, Wenzel S
    J Allergy Clin Immunol, 2015;135(1):92-9.
    Species: Human
    Sample Types: BALF
  14. Reduced IL-33 plasma levels in aplastic anemia.
    Authors: Sun M, Ma H, Che Y, Cui X
    Cancer Cell Int, 2015;15(0):118.
    Species: Human
    Sample Types: Plasma
  15. IL1RL1 gene variants and nasopharyngeal IL1RL-a levels are associated with severe RSV bronchiolitis: a multicenter cohort study.
    Authors: Faber TE, Schuurhof A, Vonk A, Koppelman GH, Hennus MP, Kimpen JL, Janssen R, Bont LJ
    PLoS ONE, 2012;7(5):e34364.
    Species: Human
    Sample Types: Tissue Secretion
  16. IL-33 mediates inflammatory responses in human lung tissue cells.
    Authors: Yagami A, Orihara K, Morita H
    J. Immunol., 2010;185(10):5743-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. High Levels of Soluble ST2 and Low Levels of IL-33 in Sera of Patients with HIV Infection.
    Authors: Miyagaki T, Sugaya M
    J. Invest. Dermatol., 2010;131(0):794.
    Species: Human
    Sample Types: Serum

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Reviews for Human ST2/IL-33R Quantikine ELISA Kit

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Human ST2/IL-33 R Quantikine ELISA Kit
By Anonymous on 12/01/2016
Sample Tested: Human serum

Human ST2/IL-1 R4 Quantikine ELISA Kit
By Anonymous on 07/26/2016
Sample Tested: EDTA Plasma,Cell culture supernatant,Cell Lysates