Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human ST3GAL2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1
    Accession # Q11201
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 by Western Blot. Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal alpha -2,3-Sialyltransferase 1/
ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1

ST3 beta -Gal alpha -2,3-Sialyltransferase 1 (ST3GAL1, also known as SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The full length ST3GAL1is a type II Golgi membrane glycoprotein that can be proteolytically processed to a soluble form. ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.

  • Long Name:
    ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1
  • Entrez Gene IDs:
    6482 (Human); 20442 (Mouse); 362924 (Rat)
  • Alternate Names:
    Alpha 2,3-ST 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1; DKFZp666E036; DKFZp779K2051; EC 2.4.99; EC 2.4.99.4; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; Gal-NAc6S; sialyltransferase 4A (beta-galactosidase alpha-2,3-sialytransferase); sialyltransferase 4A (beta-galactoside alpha-2,3-sialyltransferase); sialyltransferase 4A (beta-galactoside alpha-2,3-sialytransferase); Sialyltransferase 4A; SIAT4; SIAT4A; SIAT4-A; SIATFL; SIATFLFLJ36548; ST3 beta-galactoside alpha-2,3-sialyltransferase 1; ST3Gal I; ST3GAL1; ST3GalA; ST3GalA.1MGC9183; ST3GalI; ST3GalIA; ST3GalIA1,Beta-galactoside alpha-2,3-sialyltransferase 1; ST3O; ST3OST3GalA
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 17  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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