Detection of Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 by Western Blot.
Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ST3 beta -Gal alpha -2,3-Sialyltransferase 1 (ST3GAL1, also known as
SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that
catalyzes the transfer of sialic acid from CMP-sialic acid to
galactose-containing substrates. The full length ST3GAL1is a type II Golgi
membrane glycoprotein that can be proteolytically processed to a soluble form.
ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T
antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is
altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have
been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.
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