Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Primary Antibodies »

Product Specifications

Immunogen

human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1
Accession # Q11201

Specificity

Detects human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human ST3GAL2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Detection of Human ST3 beta -Gal a-2,3-Sialyltransferase 1/ST3GAL1 antibody by Western Blot.

Detection of Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 by Western Blot.

Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta -Gal a-2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal a-2,3-Sialyltransferase 1/ ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human placenta

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1

ST3 beta -Gal alpha -2,3-Sialyltransferase 1 (ST3GAL1, also known as SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The full length ST3GAL1is a type II Golgi membrane glycoprotein that can be proteolytically processed to a soluble form. ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1

Alternate Names

Gal-NAc6S, SIAT4A, SIATFL, ST3GalA, ST3O

Entrez Gene IDs

6482 (Human); 20442 (Mouse); 362924 (Rat)

Gene Symbol

ST3GAL1

UniProt

Q11201

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Products

Product Documents for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

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