Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 by Western Blot. Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta -Gal a-2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal a-2,3-Sialyltransferase 1/ ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1

ST3 beta -Gal alpha -2,3-Sialyltransferase 1 (ST3GAL1, also known as SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The full length ST3GAL1is a type II Golgi membrane glycoprotein that can be proteolytically processed to a soluble form. ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.

Product Datasheets

Related Research Areas

• Glycobiology-related Enzymes
• Search Glycobiology-related Enzymes