Human Syntaxin 6 Biotinylated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Syntaxin 6 in HeLa Human Cell Line. Syntaxin 6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5664) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to Golgi bodies. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Background: Syntaxin 6

Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6.

Product Datasheets

Related Research Areas

• Synaptic SNAREs