Detection of Human Syntaxin 6 by Western Blot. Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 6 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Syntaxin 6 in Human Brain. Syntaxin 6 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Syntaxin 6 in HeLa Human Cell Line. Syntaxin 6 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to Golgi vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 6
Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6.
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