Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all t-Plasminogen Activator/tPA Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human t-Plasminogen Activator/tPA
Ser36-Pro562
Accession # P00750

Specificity

Detects human t-Plasminogen Activator/tPA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human t‑Plasminogen Activator/tPA Antibody

Detection of Human t-Plasminogen Activator/tPA antibody by Western Blot.

Detection of Human t‑Plasminogen Activator/tPA by Western Blot.

Western blot shows lysates of HEK001 human epidermal keratinocyte cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human t-Plasminogen Activator/ tPA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7449) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for t-Plasminogen Activator/tPA at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human t‑Plasminogen Activator/tPA Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HEK001 human epidermal keratinocyte cell line and NTera‑2 human testicular embryonic carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using AF7449 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: t-Plasminogen Activator/tPA

PLAT (Plasminogen activator; also tPA/Tissue Plasminogen Activator) is a 64-69 kDa extracellular glycoprotein that belongs to the peptidase S1 family of enzymes. It is secreted by diverse cell types such as fibroblasts, endothelial cells and astrocytes. Unlike many other serine proteases, PLAT/tPA circulates as an active proenzyme. Following clot formation, both tPA and its substrate, plasminogen, bind to clot fibrin. Here, tPA converts plasminogen to plasmin, an enzyme that subsequently degrades the fibrin matrix of the clot. Plasmin also cleaves local tPA, generating a disulfide-linked, two-chain tPA isoform whose activity is potentiated 5- to10-fold relative to the uncleaved form. tPa also cleaves PDGF-CC and activates MMP-9, suggesting an expanded role in vascular remodeling. Human PLAT/tPA is secreted as a 530 amino acid (aa) single chain polypeptide (aa 33-562). Depending upon its source, aa 33-35 or 33-38 may be removed in the circulation. PLAT contains one N-terminal fibronectin type I region (aa 39-81), an EGD-like domain (aa 82-120), a two kringle structures (127-208; 215-296) and a peptidase S1 domain (311-561). Plasmin cleaves PLAT/tPA between Arg310-Ile311, generating an active disulfide-linked heterodimeric molecule that contains chains of 35 kDa and 33 kDa, respectively. There are multiple isoform variants. One contains a three aa substitution for aa 1-40 coupled to a deletion of aa 79-208, a second shows a Gly substitution for aa 39-85, and a third possesses a 23 aa substitution for aa 269-562. Two additional isoforms shows a His substitution for aa 54-134, and a 25 aa substitution for aa 269-562, respectively. Over aa 36-562, human PLAT shares 81% aa sequence identity with mouse PLAT.

Long Name

Tissue Plasminogen Activator

Alternate Names

Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator

Entrez Gene IDs

5327 (Human); 18791 (Mouse); 25692 (Rat)

Gene Symbol

PLAT

UniProt

P00750

Additional t-Plasminogen Activator/tPA Products

Product Documents for Human t‑Plasminogen Activator/tPA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human t‑Plasminogen Activator/tPA Antibody

For research use only

Citations for Human t‑Plasminogen Activator/tPA Antibody

Customer Reviews for Human t‑Plasminogen Activator/tPA Antibody (1)

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  • Human t-Plasminogen Activator/tPA Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 05/27/2022
    Human t‑Plasminogen Activator/tPA Antibody AF7449

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