Detection of TEM5/GPR124 in HEK293 Human Cell Line Transfected with Human TEM5/GPR124 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human TEM5/GPR124 and eGFP was stained with either (A) Mouse Anti-Human TEM5/GPR124 APC‑conjugated Monoclonal Antibody (Catalog # FAB8896A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
G protein-coupled receptor 124 (GPR124), also known as TEM5, is a member of the receptor #2 family of G protein coupled receptors, and is localized on the surface of endothelial cells. GPR124 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. This receptor mediates endothelial cell survival during angiogenesis by linking integrins to glycosaminoglycans. GPR124 has also been shown to interact with DLG1 through its PDZ-binding motif. Over amino acids 1-699, human and mouse GPR124 share 91% amino acid sequence identity.
Have you used Human TEM5/GPR124 APC-conjugated Antibody?
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