Human TEM5/GPR124 APC-conjugated Antibody

FAB8896A has been discontinued. View all TEM5/GPR124 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TEM5/GPR124 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 499716
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human TEM5/GPR124
    Met1-Leu699
    Accession # Q96PE1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TEM5/GPR124 in HEK293 Human Cell Line Transfected with Human TEM5/GPR124 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TEM5/GPR124 and eGFP was stained with either (A) Mouse Anti-Human TEM5/GPR124 APC‑conjugated Monoclonal Antibody (Catalog # FAB8896A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TEM5/GPR124
G protein-coupled receptor 124 (GPR124), also known as TEM5, is a member of the receptor #2 family of G protein coupled receptors, and is localized on the surface of endothelial cells. GPR124 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. This receptor mediates endothelial cell survival during angiogenesis by linking integrins to glycosaminoglycans. GPR124 has also been shown to interact with DLG1 through its PDZ-binding motif. Over amino acids 1-699, human and mouse GPR124 share 91% amino acid sequence identity.
  • Long Name:
    Tumor Endothelial Marker 5
  • Entrez Gene IDs:
    25960 (Human); 78560 (Mouse)
  • Alternate Names:
    DKFZp434C211; DKFZp434J0911; G protein-coupled receptor 124; GPR124; G-protein coupled receptor 124; KIAA1531; KIAA1531FLJ14390; TEM5; TEM5Tumor endothelial marker 5
Related Research Areas

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