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Human TEM5/GPR124 APC-conjugated Antibody

Catalog #: FAB8896A Datasheet / COA / SDS

Discontinued Product

FAB8896A has been discontinued.
View all TEM5/GPR124 products.
Detection of TEM5/GPR124 in HEK293 Human Cell Line Transfected with Human TEM5/GPR124 and eGFP by Flow Cytometry.
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Human TEM5/GPR124 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TEM5/GPR124 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 499716
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TEM5/GPR124
Met1-Leu699
Accession # Q96PE1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TEM5/GPR124 antibody in HEK293 Human Cell Line Transfected with Human TEM5/GPR124 and eGFP antibody by Flow Cytometry. View Larger

Detection of TEM5/GPR124 in HEK293 Human Cell Line Transfected with Human TEM5/GPR124 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TEM5/GPR124 and eGFP was stained with either (A) Mouse Anti-Human TEM5/GPR124 APC-conjugated Monoclonal Antibody (Catalog # FAB8896A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TEM5/GPR124

G protein-coupled receptor 124 (GPR124), also known as TEM5, is a member of the receptor #2 family of G protein coupled receptors, and is localized on the surface of endothelial cells. GPR124 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. This receptor mediates endothelial cell survival during angiogenesis by linking integrins to glycosaminoglycans. GPR124 has also been shown to interact with DLG1 through its PDZ-binding motif. Over amino acids 1-699, human and mouse GPR124 share 91% amino acid sequence identity.

Long Name
Tumor Endothelial Marker 5
Entrez Gene IDs
25960 (Human); 78560 (Mouse)
Alternate Names
DKFZp434C211; DKFZp434J0911; G protein-coupled receptor 124; GPR124; G-protein coupled receptor 124; KIAA1531; KIAA1531FLJ14390; TEM5; TEM5Tumor endothelial marker 5

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