|Detection of Human TGIF1 by Western Blot. Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human TGIF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7555) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TGIF1 at approximately 32-34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
TGIF1 (5'-TG[TCA]-3' Interacting Protein 1; also Transforming growth factor beta-induced factor and HPE4) is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa identity with mouse TGIF1.
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