Human TGIF1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TGIF1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TGIF1
    Lys245-Ser359
    Accession # Q15583
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TGIF1 by Western Blot. Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human TGIF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7555) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TGIF1 at approximately 32-34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGIF1

TGIF1 (5'-TG[TCA]-3' Interacting Protein 1; also Transforming growth factor beta-induced factor and HPE4) is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa identity with mouse TGIF1.

  • Long Name:
    TGFB-induced Factor Homeobox 1
  • Entrez Gene IDs:
    7050 (Human); 21815 (Mouse); 316742 (Rat)
  • Alternate Names:
    5'-TG-3' interacting factor; homeobox protein TGIF; homeobox protein TGIF1; HPE4; MGC39747; TALE homeobox TG-interacting factor; TGFB-induced factor (TALE family homeobox); TGFB-induced factor homeobox 1; TGIF1; TGIFMGC5066,5'-TG-3'-interacting factor 1; transforming growth factor-beta-induced factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells.
    Authors: Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O
    Mol Oncol, 2014;8(1):68-82.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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