Human Thioredoxin Reductase 2/TRXR2 Antibody

Detection of Human, Mouse, and Rat Thioredoxin Reductase 2/TRXR2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Thioredoxin Reductase 2/TRXR2 Monoclonal Antibody (Catalog # MAB58151) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin Reductase 2/TRXR2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Thioredoxin Reductase 2/TRXR2 in Human Prostate. Thioredoxin Reductase 2/TRXR2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Thioredoxin Reductase 2/TRXR2 Monoclonal Antibody (Catalog # MAB58151) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.