Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all THSD1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human THSD1 Isoform 2
Glu25-Ile361
Accession # Q9NS62

Specificity

Detects human THSD1 Isoform 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse THSD1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human THSD1 Isoform 2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human THSD1 Isoform 2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: THSD1

THSD1 (thrombospondin type 1 domain‑containing protein 1; also TMTSP) is a 90‑100 kDa type I transmembrane protein that is reminiscent of Unc5h proteins. It is expressed on embryonic endothelial and hematopoietic stem cells and may be involved in cell‑cell interactions. Mature human THSD1 is 827 amino acids (aa) in length. It contains a 388 aa extracellular domain (ECD) (aa 25‑412) and a 418 aa cytoplasmic region (aa 434‑851). The ECD possesses three Ig‑like domains
(aa 156‑316) and one TSP type‑1 domain (aa 340‑393). There is one potential alternate start site at Met380 and two splice variants, one of which shows a deletion of aa 341‑393 and a second that shows a 36 aa substitution for aa 394‑851, generating a soluble form. Over aa 25‑361, human THSD1 is 76% aa identical to mouse THSD1.

Long Name

Thrombospondin, Type I, Domain Containing 1

Alternate Names

TMTSP

Entrez Gene IDs

55901 (Human); 56229 (Mouse); 364630 (Rat)

Gene Symbol

THSD1

UniProt

Q9NS62

Additional THSD1 Products

Product Documents for Human THSD1 Isoform 2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human THSD1 Isoform 2 Antibody

For research use only

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