Human Thymidine Kinase 1 Antibody

Catalog # Availability Size / Price Qty
Thymidine Kinase 1 in A431 Human Cell Line.
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Product Details
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Human Thymidine Kinase 1 Antibody Summary

Species Reactivity
Detects human Thymidine Kinase 1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 1008515
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Thymidine Kinase 1
Accession # P04183
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Thymidine Kinase 1 antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

Thymidine Kinase 1 in A431 Human Cell Line. Thymidine Kinase 1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Thymidine Kinase 1 Monoclonal Antibody (Catalog # MAB8180) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thymidine Kinase 1

Thymidine kinases are 2'-deoxythymidine kinases that phosphorylate deoxythymidine and generate deoxythymidine 5’-phosphate (1). Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Two forms of thymidine kinase are present in mammalian cells, TK1 and TK2. TK1 is cell cycle-dependent and is present in the cytoplasm only in anticipation of cell division (2, 3); whereas TK2 is located in mitochondria and is cell cycle-independent (4). TK1 is synthesized by the cell during the S phase of cell division and is degraded after cell division is completed (5). TK1 normally occurs in tissue as a dimer and can be converted to more active tetrameric form by ATP binding (6). TK1 is feedback inhibited by thymidine triphosphate, the product of the further phosphorylation of thymidine (7). Because tumor cells replicate much more frequently than normal cells and requires faster DNA synthesis and higher TK1 activity, TK1 is a cancer maker especially for hematologic malignancies (8, 9). In clinical chemistry TK1 is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease (10). In addition, thymidine kinase is required for the action of many antiviral drugs (11). The enzymatic activity of recombinant human TK1 is measured using a phosphatase-coupled method (12).

  1. Wintersberger, E. (1997) Biochem. Soc. Trans. 25:303.
  2. Littelfield, J.W. (1966) Biochim. Biophys. Acta 114:398.
  3. Flemington, E, et al. (1987) Gene 52:267.
  4. Berk, A.J. et al. (1973) Arch. Biochem. Biophys. 154:563.
  5. Zhu, C. et al. (2006) Nucleosides Nucleotides Nucleic Acids 25:1185.
  6. Munch-Petersen, B. et al. (1993) J. Biol. Chem. 268:15621.
  7. Mikkelsen, N. E. et al. (2003). Biochemistry 42:5706.
  8. Doi S, et al. (1990) Nagoya J. Med. Sci. 52:19.
  9. Ellims PH, et al. (1981). Cancer Res. 41: 691.
  10. Lin TS, et al. (1976) J. Med. Chem.19:495.
  11. Baba M, et al. (1987). Biochem. Biophys. Res. Commun. 142:128.
  12. Wu, Z.L. (2011) PLoS One 6:e23172.
Entrez Gene IDs
7083 (Human); 21877 (Mouse)
Alternate Names
EC; thymidine kinase 1 soluble isoform; Thymidine Kinase 1; thymidine kinase 1, soluble; thymidine kinase, cytosolic; thymidine kinase-1; TK1; TK2

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