Human Thymopoietin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF843
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Human Thymopoietin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Thymopoietin in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Thymopoietin
Pro2-Gln187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Thymopoietin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thymopoietin

Thymopoietin (TP alpha; also TMPO, LAP2 alpha and TPRP alpha ) is a 75 kDa member of the LEM family of molecules.  It is a nucleoplasmic protein that is widely expressed in cells such as keratinocytes and thymocytes.  Here, it interacts with A-type lamins and Rb/retinoblastoma protein, thus participating in cell cycle exit.  Human thymopoietin is 694 amino acids (aa) in length.  It contains a potential N-terminal “thymopoietin peptide” segment (aa #2-49), a BAF-binding LEM domain (aa #109-153), an NLS (aa #190-196), and a coiled-coil region (aa #558-657).  Multiple splice variants exist.  All contain the same 188 N-terminal amino acids.  Five variants show singular 49, 157, 266, 226, and 60 aa substitutions for the C-terminal 506 amino acids.  Over aa #2-187, human thymopoietin shares 93% aa identity with mouse thymopoietin.

Entrez Gene IDs
7112 (Human)
Alternate Names
CMD1T; lamina-associated polypeptide 2; LAP2PRO0868; LEM domain containing 4; LEMD4; MGC61508; Thymopoietin isoform alpha; Thymopoietin; Thymopoietin, isoforms beta/gamma; Thymopoietin-related peptide isoform alpha; Thymopoietin-related peptide isoforms beta/gamma; TMPO; TP alpha; TP beta/gamma; TP; TPRP isoform alpha; TPRP isoforms beta/gamma

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