Detection of Human Thymosin beta 10 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Thymosin beta 10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6429) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 10 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thymosin beta 10
Thymosin beta 10 (T beta 10) is a 6.5 kDa member of the beta -thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 5.0-7.0. Multiple cell types produce T beta 10, either constitutively, or after stimulation. They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types. T beta 10 is principally an intracellular molecule. It forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. It is also known to be noncanonically secreted, acting on endothelium and blocking angiogenesis. Mature human T beta 10 is 43 aa in length (aa 2-44) (SwissProt #:P63313). It contains an actin-binding site (aa 18-23), an N-terminal acetylated alanine, four acetylated lysines (#4; 15; 26; 39), and one phosphorylation site at Thr23. Mature T beta 10 is identical to mouse T beta 10 in aa sequence, and it shares 74% aa sequence identity with its human family member T beta 4.
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