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OR

Human TIGIT APC‑conjugated Antibody

  • Specificity
    Detects human TIGIT in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse TIGIT is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 741182
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIGIT
    Met1-Pro141
    Accession # Q495A1
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TIGIT in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human TIGIT APC-conjugated Monoclonal Antibody (Catalog # FAB7898A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of TIGIT in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human TIGIT APC-conjugated Monoclonal Antibody (Catalog # FAB7898A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University Cell Adhesion Molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation sites, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).

  • References:
    1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
    2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
    3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
    4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
    5. SwissProt Accession # Q495A1.
    6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
    7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
    8. Joller, N. et al. (2011) J. Immunol. 83:1338.
  • Long Name:
    T Cell Immunoreceptor with Ig and ITIM Domains
  • Entrez Gene IDs:
    201633 (Human); 100043314 (Mouse); 363784 (Rat);
  • Alternate Names:
    VSIG9; VSTM3; WUCAM
Related Research Areas

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