Detection of Human Tiki2/TRABD2B by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human Tiki2/TRABD2B. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tiki2/TRABD2B at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Tiki2/TRABD2B in HEK293 Human Cell Line Transfected with Human Tiki2/TRABD2B by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either human Tiki2/ TRABD2B (filled histogram) or irrelevant transfectants (open histogram) was stained with Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TIKI2, encoded by the TRABD2B gene, is a 517 aminoacid metalloproteinase that acts as a negative regulator of Wnt signaling by mediating the cleavage of N-terminal residues in many of Wnt proteins. Tiki matalloproteins are dependent on Mn2+/Co2+ and are inhibited by divalent metal chelators like EDTA. The other known Tiki protein in human, Tiki1, is required for head formation via Wnt cleavage-oxidation and inactivation, but lacks known metalloprotease motifs. TIKI2 is upregulated in renal cell carcinoma and it appears to suppress growth of osteosarcoma by targeting the canonical Wnt pathway
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