Human TIM-3 Alexa Fluor® 700-conjugated Antibody

Clone 344823 was used by HLDA to establish CD designation.
(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
  • Source
    Monoclonal Rat IgG2A Clone # 344823
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIM‑3
    Accession # Q8TDQ0.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TIM‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mono­nuclear cells (PBMCs) were stained with Rat Anti-Human TIM‑3 Alexa Fluor® 700‑conjugated Mono­clonal Antibody (Catalog # FAB2365N) and Mouse Anti-Human CD14 PE‑conjugated Mono­clonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).

  • References:
    1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
    2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
    3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
    4. Monney, L. et al. (2002) Nature 415:536.
    5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
    6. Khademi, M. et al. (2004) J. Immunol. 172:7169.
    7. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
    8. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
    9. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
    10. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
    11. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
    12. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
    13. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
    14. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
    15. Geng, H. et al. (2006) J. Immunol. 176:1411.
  • Long Name:
    T Cell Immunoglobulin Mucin-3
  • Entrez Gene IDs:
    84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
  • Alternate Names:
    CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; Tim-3; TIMD-3; TIMD3KIM-3
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
    Authors: Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, Wong D, Busch M, Norris P, Ndhlovu L
    PLoS ONE, 2014;9(3):e92134.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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