Human TIM-3 PE-conjugated Antibody

Clone 344823 was used by HLDA to establish CD designation.
(21 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
  • Source
    Monoclonal Rat IgG2A Clone # 344823
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIM‑3
    Ser22-Arg200
    Accession # Q8TDQ0.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB2365P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-3
TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).
  • References:
    1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
    2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
    3. Monney, L. et al. (2002) Nature 415:536.
    4. Nakayama, M. et al. (2009) Blood 113:3821.
    5. Anderson, A.C. et al. (2007) Science 318:1141.
    6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
    7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
    8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
    9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
    10. Gleason, M.K. et al. (2012) Blood 119:3064.
    11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
    12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
    13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
    14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
  • Long Name:
    T Cell Immunoglobulin Mucin-3
  • Entrez Gene IDs:
    84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
  • Alternate Names:
    CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD-3; TIMD3KIM-3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency
    Authors: M Allard, B Couturaud, L Carretero-, MN Duong, J Schmidt, GC Monnot, P Romero, DE Speiser, M Hebeisen, N Rufer
    JCI Insight, 2017;2(14):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of ?? T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea
    Authors: L Schofield, LJ Ioannidis, S Karl, LJ Robinson, QY Tan, DP Poole, I Betuela, DL Hill, PM Siba, DS Hansen, I Mueller, EM Eriksson
    BMC Med, 2017;15(1):114.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
    Authors: D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. BCG vaccination induces HIV target cell activation in HIV-exposed infants in a randomized trial
    Authors: MA Gasper, AC Hesseling, I Mohar, L Myer, T Azenkot, JS Passmore, W Hanekom, MF Cotton, IN Crispe, DL Sodora, HB Jaspan
    JCI Insight, 2017;2(7):e91963.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
    Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016;126(8):3130-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients
    Sci Rep, 2016;6(0):25567.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
    Authors: Frederiksen J, Buggert M, Noyan K, Nowak P, Sonnerborg A, Lund O, Karlsson A
    PLoS ONE, 2015;10(9):e0137635.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
    Authors: Meggyes, Matyas, Miko, Eva, Polgar, Beata, Bogar, Barbara, Farkas, Balint, Illes, Zsolt, Szereday, Laszlo
    PLoS ONE, 2014;9(3):e92371.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
    Authors: Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
    PLoS ONE, 2014;9(2):e88327.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
    Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
    J Immunol, 2014;192(2):782-91.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals.
    Authors: Kared H, Saeed S, Klein M, Shoukry N
    PLoS ONE, 2014;9(7):e101441.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
    Authors: Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, Wong D, Busch M, Norris P, Ndhlovu L
    PLoS ONE, 2014;9(3):e92134.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. The immunomodulatory effects of bevacizumab on systemic immunity in patients with metastatic melanoma.
    Authors: Mansfield A, Nevala W, Lieser E, Leontovich A, Markovic S
    Oncoimmunology, 2013;2(5):e24436.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Age-related expansion of Tim-3 expressing T cells in vertically HIV-1 infected children.
    Authors: Tandon, Ravi, Giret, Maria T, Sengupta, Devi, York, Vanessa, Wiznia, Andrew A, Rosenberg, Michael, Kallas, Esper G, Ndhlovu, Lishomwa, Nixon, Douglas
    PLoS ONE, 2012;7(9):e45733.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
    Authors: Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
    PLoS ONE, 2012;7(2):e30852.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Antigen-independent induction of Tim-3 expression on human T cells by the common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.
    Authors: Mujib S, Jones RB, Lo C, Aidarus N, Clayton K, Sakhdari A, Benko E, Kovacs C, Ostrowski MA
    J. Immunol., 2012;188(8):3745-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012;7(10):e47648.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Tim-3 Negatively Regulates Cytotoxicity in Exhausted CD8(+) T Cells in HIV Infection.
    Authors: Sakhdari A, Mujib S, Vali B
    PLoS ONE, 2012;7(7):e40146.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
    Authors: Idali F, Wahlstrom J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
    Respir Res, 2009;10(0):42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 21 Citations
Isotype Controls
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Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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