Human TIM-3 PE-conjugated Antibody

Clone 344823 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2365P
Human TIM-3 PE-conjugated Antibody in Data
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Citations (26)
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Human TIM-3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
Source
Monoclonal Rat IgG2A Clone # 344823
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TIM-3 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM-3 PE-conjugated Monoclonal Antibody (Catalog # FAB2365P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References
  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Monney, L. et al. (2002) Nature 415:536.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

Product Datasheets

Citations for Human TIM-3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
    Authors: M Meggyes, L Szereday, N Bohonyi, M Koppan, S Szegedi, A Marics-Kut, M Marton, A Totsimon, B Polgar
    Int J Mol Sci, 2020;21(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors
    Authors: T Duhen, R Duhen, R Montler, J Moses, T Moudgil, NF de Miranda, CP Goodall, TC Blair, BA Fox, JE McDermott, SC Chang, G Grunkemeie, R Leidner, RB Bell, AD Weinberg
    Nat Commun, 2018;9(1):2724.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Phase I trial of plerixafor combined with decitabine in newly diagnosed older patients with acute myeloid leukemia
    Authors: GJ Roboz, EK Ritchie, Y Dault, L Lam, DC Marshall, NM Cruz, HC Hsu, DC Hassane, PJ Christos, C Ippoliti, JM Scandura, ML Guzman
    Haematologica, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of ?? T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea
    Authors: L Schofield, LJ Ioannidis, S Karl, LJ Robinson, QY Tan, DP Poole, I Betuela, DL Hill, PM Siba, DS Hansen, I Mueller, EM Eriksson
    BMC Med, 2017;15(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
    Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
    Authors: D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells
    Authors: C Stecher, C Battin, J Leitner, M Zettl, K Grabmeier-, C Höller, GJ Zlabinger, P Steinberge
    Front Immunol, 2017;8(0):572.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. BCG vaccination induces HIV target cell activation in HIV-exposed infants in a randomized trial
    Authors: MA Gasper, AC Hesseling, I Mohar, L Myer, T Azenkot, JS Passmore, W Hanekom, MF Cotton, IN Crispe, DL Sodora, HB Jaspan
    JCI Insight, 2017;2(7):e91963.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency
    Authors: M Allard, B Couturaud, L Carretero-, MN Duong, J Schmidt, GC Monnot, P Romero, DE Speiser, M Hebeisen, N Rufer
    JCI Insight, 2017;2(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016;126(8):3130-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients
    Sci Rep, 2016;6(0):25567.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
    Authors: Frederiksen J, Buggert M, Noyan K, Nowak P, Sonnerborg A, Lund O, Karlsson A
    PLoS ONE, 2015;10(9):e0137635.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
    Authors: Meggyes, Matyas, Miko, Eva, Polgar, Beata, Bogar, Barbara, Farkas, Balint, Illes, Zsolt, Szereday, Laszlo
    PLoS ONE, 2014;9(3):e92371.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
    Authors: Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, Wong D, Busch M, Norris P, Ndhlovu L
    PLoS ONE, 2014;9(3):e92134.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
    Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
    J Immunol, 2014;192(2):782-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
    Authors: Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
    PLoS ONE, 2014;9(2):e88327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals.
    Authors: Kared H, Saeed S, Klein M, Shoukry N
    PLoS ONE, 2014;9(7):e101441.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. The immunomodulatory effects of bevacizumab on systemic immunity in patients with metastatic melanoma.
    Authors: Mansfield A, Nevala W, Lieser E, Leontovich A, Markovic S
    Oncoimmunology, 2013;2(5):e24436.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
    Authors: Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
    PLoS ONE, 2012;7(2):e30852.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Age-related expansion of Tim-3 expressing T cells in vertically HIV-1 infected children.
    Authors: Tandon, Ravi, Giret, Maria T, Sengupta, Devi, York, Vanessa, Wiznia, Andrew A, Rosenberg, Michael, Kallas, Esper G, Ndhlovu, Lishomwa, Nixon, Douglas
    PLoS ONE, 2012;7(9):e45733.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012;7(10):e47648.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Antigen-independent induction of Tim-3 expression on human T cells by the common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.
    Authors: Mujib S, Jones RB, Lo C, Aidarus N, Clayton K, Sakhdari A, Benko E, Kovacs C, Ostrowski MA
    J. Immunol., 2012;188(8):3745-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Tim-3 Negatively Regulates Cytotoxicity in Exhausted CD8(+) T Cells in HIV Infection.
    Authors: Sakhdari A, Mujib S, Vali B
    PLoS ONE, 2012;7(7):e40146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
    Authors: Idali F, Wahlstrom J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
    Respir Res, 2009;10(0):42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Dendritic cell vaccination in combination with docetaxel for patients with metastatic castration-resistant prostate cancer: A randomized phase II study.
    Authors: Kongsted P, Borch T, Ellebaek E, Iversen T, Andersen R, Met O, Hansen M, Lindberg H, Sengelov L, Svane I
    Cytotherapy, 0;19(4):500-513.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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