Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 344823
View all TIM-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0.2

Specificity

Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human TIM‑3 APC‑conjugated Antibody

Detection of TIM‑3 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Human TIM-3 by Flow Cytometry

Detection of Human TIM-3 by Flow Cytometry

Antigen Expression in AML and Normal Hematopoiesis. Antigen expression on primary AML samples at initial diagnosis and healthy donor-derived bone marrow cell populations (HD BM) as indicated in legend. a Representative primary AML sample and healthy donor-derived bone marrow sample. Histograms indicate fluorescence intensity. Numbers indicate MFI ratio. b Antigen expression in AML and normal hematopoiesis, shown as MFI ratio. Dots indicate measured samples. Violin plots illustrate distribution of antigen expression for each analyzed cell population. Black dotted line indicates MFI ratio of 1.5 as cutoff for positivity. c Summary of antigen expression levels (median MFI ratio) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29946192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TIM‑3 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 3 reviews rated 4.3 using FAB2365A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

References

  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  6. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  7. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  8. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  9. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Gene Symbol

HAVCR2

UniProt

Q8TDQ0.2

Additional TIM-3 Products

Product Documents for Human TIM‑3 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TIM‑3 APC‑conjugated Antibody

For research use only

Citations for Human TIM‑3 APC‑conjugated Antibody

Customer Reviews for Human TIM‑3 APC‑conjugated Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human TIM‑3 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Human TIM-3 APC-conjugated Antibody
    Name: Vikram Mavinkurve
    Application: Flow Cytometry
    Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC)
    Species: Human
    Verified Customer | Posted 05/10/2018
    Human PBMCs were stimulated with anti-human CD3(1ug/ml) for 48hrs and stained with Isotype and test antibodies. The results have been consistent and reliable.
    Human TIM‑3 APC‑conjugated Antibody FAB2365A
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24465502
    Species: Human
    Verified Customer | Posted 01/22/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22475535
    Species: Human
    Verified Customer | Posted 01/22/2015

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies