Human TIM-3 APC-conjugated Antibody

Clone 344823 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry.
1 Image
Product Details
Citations (10)
Supplemental Products
Reviews (1)

Human TIM-3 APC-conjugated Antibody Summary

Species Reactivity
Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
Monoclonal Rat IgG2A Clone # 344823
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Accession # Q8TDQ0.2
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TIM‑3 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  6. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  7. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  8. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  9. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

Product Datasheets

You must select a language.


Citations for Human TIM-3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. CD226 opposes TIGIT to disrupt Tregs in melanoma
    Authors: J Fourcade, Z Sun, JM Chauvin, M Ka, D Davar, O Pagliano, H Wang, S Saada, C Menna, R Amin, C Sander, JM Kirkwood, AJ Korman, HM Zarour
    JCI Insight, 2018;3(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1c259T cells in Synovial Sarcoma
    Authors: SP D'Angelo, L Melchiori, MS Merchant, DB Bernstein, J Glod, RN Kaplan, SA Grupp, WD Tap, K Chagin, GK Binder, S Basu, DE Lowther, R Wang, N Bath, A Tipping, G Betts, I Ramachandr, JM Navenot, H Zhang, DK Wells, E Van Winkle, G Kari, T Trivedi, T Holdich, LN Pandite, R Amado, CL Mackall
    Cancer Discov, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Circulating T lymphocyte subsets, cytokines, and immune checkpoint inhibitors in patients with bipolar II or major depression: a preliminary study
    Authors: W Wu, YL Zheng, LP Tian, JB Lai, CC Hu, P Zhang, JK Chen, JB Hu, ML Huang, N Wei, WJ Xu, WH Zhou, SJ Lu, J Lu, HL Qi, DD Wang, XY Zhou, JF Duan, Y Xu, SH Hu
    Sci Rep, 2017;7(0):40530.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. T-cell invigoration to tumour burden ratio associated with anti-PD-1 response
    Authors: AC Huang, MA Postow, RJ Orlowski, R Mick, B Bengsch, S Manne, W Xu, S Harmon, JR Giles, B Wenz, M Adamow, D Kuk, KS Panageas, C Carrera, P Wong, F Quagliarel, B Wubbenhors, K D'Andrea, KE Pauken, RS Herati, RP Staupe, JM Schenkel, S McGettigan, S Kothari, SM George, RH Vonderheid, RK Amaravadi, GC Karakousis, LM Schuchter, X Xu, KL Nathanson, JD Wolchok, TC Gangadhar, EJ Wherry
    Nature, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. TIM-3/Gal-9 interaction induces IFNgamma-dependent IDO1 expression in acute myeloid leukemia blast cells.
    Authors: Folgiero V, Cifaldi L, Li Pira G, Goffredo B, Vinti L, Locatelli F
    J Hematol Oncol, 2015;8(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules.
    Authors: Raziorrouh B, Heeg M, Kurktschiev P, Schraut W, Zachoval R, Wendtner C, Wachtler M, Spannagl M, Denk G, Ulsenheimer A, Bengsch B, Pircher H, Diepolder H, Gruner N, Jung M
    PLoS ONE, 2014;9(8):e105703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
    Authors: Meggyes, Matyas, Miko, Eva, Polgar, Beata, Bogar, Barbara, Farkas, Balint, Illes, Zsolt, Szereday, Laszlo
    PLoS ONE, 2014;9(3):e92371.
    Species: Human
    Sample Types:
  8. Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
    Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
    J Virol, 2013;87(8):4372-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B.
    Authors: Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z
    Eur. J. Immunol., 2012;42(5):1180-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
    Authors: Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
    PLoS ONE, 2011;6(5):e19664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human TIM-3 APC-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human TIM-3 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human TIM-3 APC-conjugated Antibody
By Vikram Mavinkurve on 05/10/2018
Application: Flow Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC) Species: Human

Human PBMCs were stimulated with anti-human CD3(1ug/ml) for 48hrs and stained with Isotype and test antibodies. The results have been consistent and reliable.