Human TIM-3 Antibody
Human TIM-3 Antibody Summary
Met1-Arg200
Accession # Q8TDQ0
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-3
TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). NK cells appear to transcribe the highest amounts of Tim-3 among lymphocytes, and when Tim-3 was cross-linked with antibodies it suppressed NK cell-mediated cytotoxicity (6). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11), and regulatory T cell development (5). TIM-3 inhibits the antitumor efficacy of DNA vaccines and chemotherapy
by binding to the damage-associated molecular pattern molecule, HMGB1 (13).
- Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B. 360:1681.
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
- Monney, L. et al. (2002) Nature 415:536.
- Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
- Ndhlovu, L. et al. (2012) Blood 119:3734.
- Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
- van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
- Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
- Oikawa, T. et al. (2006) J. Immunol. 177:4281.
- Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Chiba, S. et al. (2012) Nat. Immunol. 13:832.
Product Datasheets
Citations for Human TIM-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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OX40 and LAG3 are associated with better prognosis in advanced gastric cancer patients treated with anti-programmed death-1 antibody
Authors: H Ohmura, K Yamaguchi, F Hanamura, M Ito, A Makiyama, K Uchino, H Shimokawa, S Tamura, T Esaki, K Mitsugi, Y Shibata, H Oda, K Tsuchihash, H Ariyama, H Kusaba, Y Oda, K Akashi, E Baba
Br. J. Cancer, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
J. Immunol, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
Authors: D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma
J. Leukoc. Biol., 2017;0(0):.
Species: Human
Sample Types: Plasma
Applications: Immunoprecipitation -
Human Galectin-9 Is a Potent Mediator of HIV Transcription and Reactivation
Authors: Mohamed Abdel-Mohs
PLoS Pathog, 2016;12(6):e1005677.
Species: Human
Sample Types: Whole Cells
Applications: Agonist Activity
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