TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2 is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. It is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). TIM-3 ligation by Galectin-9 attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. Dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. TIM-3 also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA.
Human TIM-3 Quantikine ELISA Kit
R&D Systems | Catalog # DTIM30
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Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
Sensitivity
8.75 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human TIM-3 Quantikine ELISA Kit
The Quantikine Human TIM-3 Immunoassay is a 2.5 hour solid phase ELISA designed to measure TIM-3 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human TIM-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TIM-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human TIM-3 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TIM-3.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human TIM-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 182 | 561 | 1132 | 184 | 549 | 1103 |
| Standard Deviation | 4.05 | 11.7 | 29.0 | 9.69 | 21.9 | 42.7 |
| CV% | 2.2 | 2.1 | 2.6 | 5.3 | 4.0 | 3.9 |
Recovery for Human TIM-3 Quantikine ELISA Kit
The recovery of human TIM-3 spiked to levels throughout the range of the assay in various matrices was evaluated
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 94-108 |
| Cell Lysis Buffer (n=1) | 102 | 96-107 |
| EDTA Plasma (n=4) | 100 | 93-105 |
| Heparin Plasma (n=4) | 97 | 92-100 |
| Serum (n=4) | 102 | 99-106 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human TIM-3 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TIM-3 Quantikine ELISA Kit
Human TIM-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-3
Long Name
T Cell Immunoglobulin Mucin-3
Alternate Names
CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3
Entrez Gene IDs
Gene Symbol
HAVCR2
Additional TIM-3 Products
Product Documents for Human TIM-3 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TIM-3 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human TIM-3 Quantikine ELISA Kit
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Protocols
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