Human TIM-3 Quantikine ELISA Kit

  
  • Assay Length
    2.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
  • Sensitivity
    8.75 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human TIM-3.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC217, Quantikine Immunoassay Control Set 887 Human TIM-3 - Please Inquire
Product Summary
The Quantikine Human TIM-3 Immunoassay is a 2.5 hour solid phase ELISA designed to measure TIM-3 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human TIM-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TIM-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human TIM-3 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TIM-3.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean18256111321845491103
Standard Deviation4.0511.7299.6921.942.7
CV%2.22.12.65.343.9

Recovery

The recovery of human TIM-3 spiked to levels throughout the range of the assay in various matrices was evaluated

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 94-108
Cell Lysis Buffer (n=1) 102 96-107
EDTA Plasma (n=4) 100 93-105
Heparin Plasma (n=4) 97 92-100
Serum (n=4) 102 99-106
Linearity
To assess the linearity of the assay, samples containing high concentrations of human TIM-3 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TIM-3 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-3
TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2 is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. It is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). TIM-3 ligation by Galectin-9 attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. Dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. TIM-3 also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA.
    • Long Name
      T Cell Immunoglobulin Mucin-3
    • Entrez Gene IDs
      84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey);
    • Alternate Names
      CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD-3; TIMD3KIM-3;
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 887 Human TIM-3

    Ctrl QC217
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Lysis Buffer 17 (1 x 21 mL)

    ELISA 895943 4
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