Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human TLR8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TLR8 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8999R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TLR8, also designated as CD288 (cluster of differentiation 288),
is a 135-140 kDa member of the Toll-like Receptor (TLR) family, TLR7/8/9 subfamily. TLRs make up a family of pattern recognition receptors that play
important roles in the innate immune response. Broad classes of pathogens (e.g.
viruses, bacteria, and fungi) constitutively express a set of
mutation-resistant molecules called pathogen-associated molecular patterns
(PAMPs). These microbial molecular markers may be composed of proteins,
carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual
TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades
that promote the immune response. Cells known to express TLR8 include dermal and myeloid dendritic cells, monocytes, CD8+ T cells and colonic columnar epithelium. Structurally, TLRs are type I transmembrane
receptors that possess varying numbers of extracellular N-terminal leucine-rich
repeat (LRR) domains, followed by a cysteine-rich region, a TM segment, and an
intracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the larger
IL-1 R/TLR superfamily. Human TLR8 is a homodimeric endosomal receptor that recognizes single
stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza,
Sendai, and Coxsackie B viruses. TLR8 binding to the viral RNA recruits MyD88
and leads to activation of the transcription factor NF-kB and an antiviral
response. Over amino acids (aa) 27-827, human and mouse TLR8 share 67% amino acid sequence identity. Notably, rodent TLR8 does not posess an RQSYA sequence between aa 438-442, rendering it unresponsive to nucleic acid sequences that lack an accompanying poly-thymidine segment.
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