Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TLR8 and eGFP was stained with and either (A) Mouse Anti-Human TLR8 Monoclonal Antibody (Catalog # MAB8999) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TLR8, also designated as CD288 (cluster of differentiation 288),
is a member of the toll-like receptor (TLR) family. TLRs make up a family of pattern recognition receptors that play
important roles in the innate immune response. Broad classes of pathogens (e.g.
viruses, bacteria, and fungi) constitutively express a set of
mutation-resistant molecules called pathogen-associated molecular patterns
(PAMPs). These microbial molecular markers may be composed of proteins,
carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual
TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades
that promote the immune response. Structurally, TLRs are type I transmembrane
receptors that possess varying numbers of extracellular N-terminal leucine-rich
repeat (LRR) motifs, followed by a cysteine-rich region, a TM domain, and an
intracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the larger
IL-1 R/TLR superfamily. Human TLR8 is an endosomal receptor that recognizes single
stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza,
Sendai, and Coxsackie B viruses. TLR8 binding to the viral RNA recruits MyD88
and leads to activation of the transcription factor NF-kB and an antiviral
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