Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry.
Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3195F) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
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