Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # TRA-1-85
View all TRA-1-85/CD147 Primary Antibodies »

Product Specifications

Immunogen

2120Ep human embryonal carcinoma cell line

Specificity

Detects human TRA‑1‑85.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRA‑1‑85/CD147 Biotinylated Antibody

TRA‑1‑85/CD147 in THP‑1 Human Cell Line.

TRA‑1‑85/CD147 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human TRA‑1‑85/CD147 Biotinylated Monoclonal Antibody (Catalog # BAM3195) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human TRA‑1‑85/CD147 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line

Western Blot

Williams, B.P. et al. (1988) Immunogenetics. 27:322. This application was not tested by R&D Systems.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TRA-1-85/CD147

The TRA-1-85 clone recognizes the cell surface expressed Oka blood group antigen in almost all human cell types (1). This antibody is frequently used to identify human cells.

References

  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.

Alternate Names

OKa Blood Group Antigen, TRA185

Entrez Gene IDs

682 (Human)

Gene Symbol

BSG

Additional TRA-1-85/CD147 Products

Product Documents for Human TRA‑1‑85/CD147 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TRA‑1‑85/CD147 Biotinylated Antibody

For research use only

Customer Reviews for Human TRA‑1‑85/CD147 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human TRA‑1‑85/CD147 Biotinylated Antibody and earn rewards!

Have you used Human TRA‑1‑85/CD147 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies