Human TRA-1-85/CD147 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Detects human TRA‑1‑85 antigen in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # TRA-1-85
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 PE‑conjugated Monoclonal Antibody (Catalog # FAB3195P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
  • References:
    1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
    2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
  • Entrez Gene IDs:
    682 (Human)
  • Alternate Names:
    OKa Blood Group Antigen; TRA185
Related Research Areas

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Sample Type
  1. Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft.
    Authors: Skrbo N, Hjortland G, Kristian A, Holm R, Nord S, Prasmickaite L, Engebraaten O, Maelandsmo G, Sorlie T, Andersen K
    PLoS ONE, 2014;9(11):e113278.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells.
    Authors: Storm MP, Orchard CB, Bone HK, Chaudhuri JB, Welham MJ
    Biotechnol. Bioeng., 2010;107(4):683-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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