Human TRA-1-85/CD147 PE-conjugated Antibody

Catalog #: FAB3195P
5 Citations Datasheet / COA / SDS

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FAB3195P

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Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-85/CD147 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRA‑1‑85 antigen in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # TRA-1-85

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

2120Ep human embryonal carcinoma cell line

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 PE-conjugated Monoclonal Antibody (Catalog # FAB3195P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

References

Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

Entrez Gene IDs

682 (Human)

Alternate Names

OKa Blood Group Antigen; TRA185; TRA-1-85

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TRA-1-85/CD147 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
Authors: EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark
Stem Cell Research, 2021;54(0):102446.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
Authors: EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark
Stem Cell Research, 2021;54(0):102409.
Species: Human
Sample Types: Whole Cells
Applications: IHC
Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research
Authors: EC Pandolfi, EJ Rojas, E Sosa, JJ Gell, TJ Hunt, S Goldsmith, Y Fan, SJ Silber, AT Clark
Stem Cell Res, 2019;40(0):101576.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft.
Authors: Skrbo N, Hjortland G, Kristian A, Holm R, Nord S, Prasmickaite L, Engebraaten O, Maelandsmo G, Sorlie T, Andersen K
PLoS ONE, 2014;9(11):e113278.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells.
Authors: Storm MP, Orchard CB, Bone HK, Chaudhuri JB, Welham MJ
Biotechnol. Bioeng., 2010;107(4):683-95.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry