Detection of Human TRA‑1‑85/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for|
TRA‑1‑85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
|Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).|
|TRA‑1‑85/CD147 in BG01V Human Embryonic Stem Cells. TRA‑1‑85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
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