Human TRA-1-85/CD147 Antibody

Catalog # Availability Size / Price Qty
MAB3195
MAB3195-SP
Detection of Human TRA‑1‑85/CD147 by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human TRA-1-85/CD147 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA‑1‑85 antigen in Western blots.
Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
TRA‑1‑85/CD147 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in TRA‑1‑85/CD147 knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human TRA-1-85/CD147 antibody by Western Blot. View Larger

Detection of Human TRA‑1‑85/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TRA‑1‑85/CD147 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry TRA-1-85/CD147 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

TRA‑1‑85/CD147 in BG01V Human Embryonic Stem Cells. TRA-1-85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human TRA-1-85/CD147 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human TRA‑1‑85/CD147 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRA-1-85/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

References
  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
  2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
Entrez Gene IDs
682 (Human)
Alternate Names
OKa Blood Group Antigen; TRA185; TRA-1-85

Product Datasheets

Citations for Human TRA-1-85/CD147 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Xeno-free cryopreservation of adherent retinal pigmented epithelium yields viable and functional cells in vitro and in vivo
    Authors: BO Pennington, JK Bailey, MA Faynus, C Hinman, MN Hee, R Ritts, V Nadar, D Zhu, D Mitra, JC Martinez-C, TC Lin, BB Thomas, DR Hinton, MS Humayun, J Lebkowski, LV Johnson, DO Clegg
    Scientific Reports, 2021;11(1):6286.
    Species: Bovine
    Sample Types: Whole Tissue
    Applications: IHC
  2. PDGFA/PDGFR?-regulated GOLM1 promotes human glioma progression through activation of AKT
    Authors: R Xu, J Ji, X Zhang, M Han, C Zhang, Y Xu, Y Wei, S Wang, B Huang, A Chen, D Zhang, Q Zhang, W Li, Z Jiang, J Wang, X Li
    J. Exp. Clin. Cancer Res., 2017;36(1):193.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth
    Authors: Ronald A DePinho
    Cell, 2016;167(5):1281-1295.e18.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Bacterially expressed full-length recombinant Plasmodium falciparum RH5 protein binds erythrocytes and elicits potent strain-transcending parasite-neutralizing antibodies.
    Authors: Reddy, K Sony, Pandey, Alok K, Singh, Hina, Sahar, Tajali, Emmanuel, Amlabu, Chitnis, Chetan E, Chauhan, Virander, Gaur, Deepak
    Infect Immun, 2014;82(1):152-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5.
    Authors: Douglas A, Williams A, Knuepfer E, Illingworth J, Furze J, Crosnier C, Choudhary P, Bustamante L, Zakutansky S, Awuah D, Alanine D, Theron M, Worth A, Shimkets R, Rayner J, Holder A, Wright G, Draper S
    J Immunol, 2014;192(1):245-58.
    Species: Human
    Sample Types: Protein
    Applications: Neutralization
  6. Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells.
    Authors: Lathia JD, Gallagher J, Myers JT
    PLoS ONE, 2011;6(9):e24807.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Human TRA-1-85/CD147 Antibody

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Human TRA-1-85/CD147 Antibody
By Anonymous on 08/30/2017
Application: IHC Sample Tested: Cardiac Progenitors Species: Human