Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca nemestrina (Southern Pig-tailed Macaque)

Applications

Validated:

Flow Cytometry

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # TRA-1-85
View all TRA-1-85/CD147 Primary Antibodies »

Product Specifications

Immunogen

2120Ep human embryonal carcinoma cell line

Specificity

Detects human TRA‑1‑85 antigen in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRA‑1‑85/CD147 APC‑conjugated Antibody

Detection of TRA-1-85/CD147 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry.

Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 APC-conjugated Monoclonal Antibody (Catalog # FAB3195A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRA‑1‑85/CD147 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes and monocytes

Reviewed Applications

Read 2 reviews rated 3 using FAB3195A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

References

  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
  2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

Alternate Names

OKa Blood Group Antigen, TRA185

Entrez Gene IDs

682 (Human)

Gene Symbol

BSG

Additional TRA-1-85/CD147 Products

Product Documents for Human TRA‑1‑85/CD147 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TRA‑1‑85/CD147 APC‑conjugated Antibody

For research use only

Citations for Human TRA‑1‑85/CD147 APC‑conjugated Antibody

Customer Reviews for Human TRA‑1‑85/CD147 APC‑conjugated Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used Human TRA‑1‑85/CD147 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human TRA-1-85/CD147 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    I use this antibody to quantify total cell numbers in human iPSC induction experiments (to avoid counting feeders cells). I use TRA-1-81 antibody to stain reprogrammed cells and TRA-1-85 antibody to stain total cells, then quantify using FACS, and use the ratio of those two numbers as reprogramming efficiency.
  • Human TRA-1-85/CD147 APC-conjugated Antibody
    Name: Jingyao Qiu
    Application: Flow Cytometry
    Sample Tested: Cells from hematopoietic differentiation of hESC line H9
    Species: Human
    Verified Customer | Posted 08/16/2016

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies