Human TRA-1-85/CD147 APC-conjugated Antibody

(9 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TRA‑1‑85 antigen in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # TRA-1-85
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 APC‑conjugated Monoclonal Antibody (Catalog # FAB3195A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
  • References:
    1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
    2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
  • Entrez Gene IDs:
    682 (Human)
  • Alternate Names:
    OKa Blood Group Antigen; TRA185; TRA-1-85
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Species
Applications
Sample Type
  1. In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Authors: P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach
    Stem Cells Int, 2016;2016(0):7513252.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor
    Authors: S Nodomi, K Umeda, S Saida, T Kinehara, T Hamabata, T Daifu, I Kato, H Hiramatsu, KI Watanabe, Y Kuwahara, T Iehara, S Adachi, E Konishi, T Nakahata, H Hosoi, T Heike
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Type: Whole Cells
    Application: Flow
  4. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
    Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
    Nat Commun, 2014;5(0):4806.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs.
    Authors: Liu G, Suzuki K, Li M, Qu J, Montserrat N, Tarantino C, Gu Y, Yi F, Xu X, Zhang W, Ruiz S, Plongthongkum N, Zhang K, Masuda S, Nivet E, Tsunekawa Y, Soligalla R, Goebl A, Aizawa E, Kim N, Kim J, Dubova I, Li Y, Ren R, Benner C, del Sol A, Bueren J, Trujillo J, Surralles J, Cappelli E, Dufour C, Esteban C, Izpisua Belmonte J
    Nat Commun, 2014;5(0):4330.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
    Nat Commun, 2014;5(0):3678.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. beta-catenin confers resistance to PI3K and AKT inhibitors and subverts FOXO3a to promote metastasis in colon cancer.
    Authors: Tenbaum SP, Ordonez-Moran P, Puig I, Chicote I, Arques O, Landolfi S, Fernandez Y, Herance JR, Gispert JD, Mendizabal L, Aguilar S, Ramon y Cajal S, Schwartz S, Vivancos A, Espin E, Rojas S, Baselga J, Tabernero J, Munoz A, Palmer HG
    Nat. Med., 2012;18(6):892-901.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Generation of red blood cells from human induced pluripotent stem cells.
    Authors: Dias J, Gumenyuk M, Kang H, Vodyanik M, Yu J, Thomson JA, Slukvin II
    Stem Cells Dev., 2011;20(9):1639-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.
    Authors: Choi KD, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M, Thomson J, Slukvin I
    Stem Cells, 2009;27(3):559-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 3 (Based on 2 reviews)

Have you used Human TRA-1-85/CD147 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human TRA-1-85/CD147 APC-conjugated Antibody.

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

Filter by Ratings
Average
(2)
All Ratings
Filter by Applications
Flow
(2)
All Applications
Filter by Species
Human
(2)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Average
 Flow Human Anonymous 12/15/2016
Show

Summary

ApplicationFlow Cytometry
Sample Testedinduced pluripotent stem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsI use this antibody to quantify total cell numbers in human iPSC induction experiments (to avoid counting feeders cells). I use TRA-1-81 antibody to stain reprogrammed cells and TRA-1-85 antibody to stain total cells, then quantify using FACS, and use the ratio of those two numbers as reprogramming efficiency.
  Average
 Flow Human Anonymous 08/16/2016
Show

Summary

ApplicationFlow Cytometry
Sample TestedCells from hematopoietic differentiation of hESC line H9
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information