Human TRA-1-85/CD147 APC-conjugated Antibody

(5 citations)
(1 Review)
  • Species Reactivity
  • Specificity
    Detects human TRA‑1‑85 antigen in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # TRA-1-85
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 APC‑conjugated Monoclonal Antibody (Catalog # FAB3195A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
  • References:
    1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
    2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
  • Entrez Gene IDs:
    682 (Human)
  • Alternate Names:
    OKa Blood Group Antigen; TRA185
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor
    Authors: S Nodomi, K Umeda, S Saida, T Kinehara, T Hamabata, T Daifu, I Kato, H Hiramatsu, KI Watanabe, Y Kuwahara, T Iehara, S Adachi, E Konishi, T Nakahata, H Hosoi, T Heike
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Macaca nemistrina
    Sample Type: Whole Cells
    Application: Flow
  3. beta-catenin confers resistance to PI3K and AKT inhibitors and subverts FOXO3a to promote metastasis in colon cancer.
    Authors: Tenbaum SP, Ordonez-Moran P, Puig I, Chicote I, Arques O, Landolfi S, Fernandez Y, Herance JR, Gispert JD, Mendizabal L, Aguilar S, Ramon y Cajal S, Schwartz S, Vivancos A, Espin E, Rojas S, Baselga J, Tabernero J, Munoz A, Palmer HG
    Nat. Med., 2012;18(6):892-901.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Generation of red blood cells from human induced pluripotent stem cells.
    Authors: Dias J, Gumenyuk M, Kang H, Vodyanik M, Yu J, Thomson JA, Slukvin II
    Stem Cells Dev., 2011;20(9):1639-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.
    Authors: Choi KD, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M, Thomson J, Slukvin I
    Stem Cells, 2009;27(3):559-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
 Flow Human Anonymous 08/16/2016


ApplicationFlow Cytometry
Sample TestedCells from hematopoietic differentiation of hESC line H9

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