Human TRAIL R1/TNFRSF10A PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB347P-100
FAB347P-025
Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (17)
FAQs
Supplemental Products
Reviews

Human TRAIL R1/TNFRSF10A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human TRAIL R1/TNFRSF10A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAIL R2, rhTRAIL R3, rhTRAIL R4, or rhDcR3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 69036
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRAIL R1/TNFRSF10A
Ala24-Asn239
Accession # AAC51226
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TRAIL R1/TNFRSF10A PE-conjugated Monoclonal Antibody (Catalog # FAB347P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL R1/TNFRSF10A

Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL RI is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References
  1. Pan, G. et al. (1997) Science 276:111.
  2. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 1
Entrez Gene IDs
8797 (Human)
Alternate Names
APO2; CD261 antigen; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4 TRAIL receptor 1; DR4; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL R1; TRAILR1; TRAIL-R1; TRAILR-1; TRAILR1MGC9365; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily, member 10a

Product Datasheets

Citations for Human TRAIL R1/TNFRSF10A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. TRAIL regulatory receptors constrain human hepatic stellate cell apoptosis
    Authors: HD Singh, I Otano, K Rombouts, KP Singh, D Peppa, US Gill, K Böttcher, PTF Kennedy, J Oben, M Pinzani, H Walczak, G Fusai, WMC Rosenberg, MK Maini
    Sci Rep, 2017;7(1):5514.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Kaempferol Sensitizes Human Ovarian Cancer Cells-OVCAR-3 and SKOV-3 to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis via JNK/ERK-CHOP Pathway and Up-Regulation of Death Receptors 4 and 5
    Authors: Y Zhao, B Tian, Y Wang, H Ding
    Med. Sci. Monit., 2017;23(0):5096-5105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
    Authors: Warat M, Sadowski T, Szliszka E, Krol W, Czuba Z
    Molecules, 2015;20(1):900-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  4. TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells.
    Authors: Tang X, Lu J, Tu H, Huang K, Fu R, Cao G, Huang M, Cheng L, Dai L, Zhang L
    Anticancer Res, 2014;34(2):729-34.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow
  5. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
    Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo
    Stem Cells Transl Med, 2014;3(2):172-82.
  6. Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages.
    Authors: Warat M, Szliszka E, Korzonek-Szlacheta I, Krol W, Czuba Z
    Int J Mol Sci, 2014;15(7):11510-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  7. Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway.
    Authors: Gupta, Subash C, Francis, Sajin K, Nair, Mangalam, Mo, Yin-Yuan, Aggarwal, Bharat B
    J Biol Chem, 2013;288(45):32343-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia.
    Authors: Gojo I, Tan M, Fang H, Sadowska M, Lapidus R, Baer M, Carrier F, Beumer J, Anyang B, Srivastava R, Espinoza-Delgado I, Ross D
    Clin Cancer Res, 2013;19(7):1838-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  9. Smad7 protein induces interferon regulatory factor 1-dependent transcriptional activation of caspase 8 to restore tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis.
    Authors: Hong S, Kim H, Kim J, Ha H, Kim Y, Bae E, Kim T, Lee K, Kim S
    J Biol Chem, 2013;288(5):3560-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  10. TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity.
    Authors: Kalb ML, Glaser A, Stary G, Koszik F, Stingl G
    J. Immunol., 2012;188(4):1583-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  11. Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response.
    Authors: Kang Z, Chen J, Yu Y, Li B, Sun S, Zhang B, Cao L
    Clin Cancer Res, 2011;17(10):3181-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  12. The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells.
    Authors: Szliszka E, Helewski KJ, Mizgala E, Krol W
    Int. J. Oncol., 2011;39(4):771-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  13. Epigenetic silencing of death receptor 4 mediates tumor necrosis factor-related apoptosis-inducing ligand resistance in gliomas.
    Authors: Elias A, Siegelin MD, Steinmuller A, von Deimling A, Lass U, Korn B, Mueller W
    Clin. Cancer Res., 2009;15(17):5457-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  14. TRAIL recombinant adenovirus triggers robust apoptosis in multidrug-resistant HL-60/Vinc cells preferentially through death receptor DR5.
    Authors: Wu CH, Kao CH, Safa AR
    Hum. Gene Ther., 2008;19(7):731-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  15. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
    Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
    Stem Cells, 2008;26(11):2955-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  16. Gene therapy using TRAIL-secreting human umbilical cord blood-derived mesenchymal stem cells against intracranial glioma.
    Authors: Kim SM, Lim JY, Park SI, Jeong CH, Oh JH, Jeong M, Oh W, Park SH, Sung YC, Jeun SS
    Cancer Res., 2008;68(23):9614-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  17. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
    Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
    J. Exp. Med., 2007;204(3):667-80.
    Species: Human
    Sample Types: Serum
    Applications: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human TRAIL R1/TNFRSF10A PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human TRAIL R1/TNFRSF10A PE-conjugated Antibody and earn rewards!

Have you used Human TRAIL R1/TNFRSF10A PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review