Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody

Detection of TRAIL R2/TNFRSF10B in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B APC-conjugated Monoclonal Antibody (Catalog # FAB6311A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TRAIL R2/TNFRSF10B by Western Blot

pHe influences the expression of TRAIL death receptors. (A,B) PDAC cells were grown for 24 h, then stained with APC-conjugated anti-TRAIL-Rs antibodies to measure their cell surface expression by flow cytometry. (C,D) PDAC cells were grown for 24 h, then either not treated (−) or treated (+) with 200 ng/ml TRAIL for 24 h, lysed, and subjected to western blot analyses for TRAIL-R1 and TRAIL-R2. Blots are representative and (E,F) show densitometric quantification normalized to loading control and the respective level of untreated cells cultured under pHe 7.4 conditions. Data are shown as mean with S.E.M error bars, of at least three independent experiments per cell line. **<0.01, ***<0.001, and ****<0.0001: significant difference between untreated and treated cells using two-way ANOVA with Sidak’s multiple-comparison test or between pHe conditions using two-way ANOVA with Tukey’s multiple-comparison test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35281089), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of TRAIL R2/TNFRSF10B by Western Blot

pHe influences the expression of TRAIL death receptors. (A,B) PDAC cells were grown for 24 h, then stained with APC-conjugated anti-TRAIL-Rs antibodies to measure their cell surface expression by flow cytometry. (C,D) PDAC cells were grown for 24 h, then either not treated (−) or treated (+) with 200 ng/ml TRAIL for 24 h, lysed, and subjected to western blot analyses for TRAIL-R1 and TRAIL-R2. Blots are representative and (E,F) show densitometric quantification normalized to loading control and the respective level of untreated cells cultured under pHe 7.4 conditions. Data are shown as mean with S.E.M error bars, of at least three independent experiments per cell line. **<0.01, ***<0.001, and ****<0.0001: significant difference between untreated and treated cells using two-way ANOVA with Sidak’s multiple-comparison test or between pHe conditions using two-way ANOVA with Tukey’s multiple-comparison test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35281089), licensed under a CC-BY license. Not internally tested by R&D Systems.