Human TRAIL R2/TNFRSF10B Antibody
Summary
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Human TRAIL R2/TNFRSF10B Antibody Summary
Species Reactivity
Human
Specificity
Detects human TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2.
Source
Monoclonal Mouse IgG2B Clone # 71908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Glu182
Accession # O14763
Ile56-Glu182
Accession # O14763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
MDA‑MB‑231 human breast cancer cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
- Chaudhary, P.M. et al. (1997) Immunity 7:821.
- Walczak, H. et al. (1997) EMBO J. 16:5386.
- Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 2
Entrez Gene IDs
8795 (Human); 21933 (Mouse)
Alternate Names
CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9
Product Datasheets
Citations for Human TRAIL R2/TNFRSF10B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
20
Citations: Showing 1 - 10
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Marizomib sensitizes primary glioma cells to apoptosis induced by a latest-generation TRAIL receptor agonist
Authors: C Boccellato, E Kolbe, N Peters, V Juric, G Fullstone, M Verreault, A Idbaih, MLM Lamfers, BM Murphy, M Rehm
Cell Death & Disease, 2021;12(7):647.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD154 inhibits death of T cells via a Cis interaction with the alpha5beta1 integrin
Authors: M Bachsais, S Salti, K Zaoui, GS Hassan, F Aoudjit, W Mourad
PLoS ONE, 2020;15(8):e0235753.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Stress-induced TRAILR2 expression overcomes TRAIL resistance in cancer cell spheroids
Authors: D Stöhr, JO Schmid, TB Beigl, A Mack, DS Maichl, K Cao, B Budai, G Fullstone, RE Kontermann, TE Mürdter, SWG Tait, C Hagenloche, N Pollak, P Scheurich, M Rehm
Cell Death Differ., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Potential Role of CD133 Expression in the Susceptibility of Human Liver Cancer Stem-Like Cells to TRAIL
Authors: SH Lee, SK Hyun, HB Kim, CD Kang, SH Kim
Oncol. Res., 2016;24(6):495-509.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells.
Authors: Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y
Int J Oncol, 2014;44(2):616-28.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dominant negative effects of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptor 4 on TRAIL receptor 1 signaling by formation of heteromeric complexes.
Authors: Neumann S, Hasenauer J, Pollak N, Scheurich P
J Biol Chem, 2014;289(23):16576-87.
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TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells.
Authors: Tang X, Lu J, Tu H, Huang K, Fu R, Cao G, Huang M, Cheng L, Dai L, Zhang L
Anticancer Res, 2014;34(2):729-34.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Casticin potentiates TRAIL-induced apoptosis of gastric cancer cells through endoplasmic reticulum stress.
Authors: Zhou Y, Tian L, Long L, Quan M, Liu F, Cao J
PLoS ONE, 2013;8(3):e58855.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Exosomes secreted by human placenta carry functional Fas ligand and TRAIL molecules and convey apoptosis in activated immune cells, suggesting exosome-mediated immune privilege of the fetus.
Authors: Stenqvist A, Nagaeva O, Baranov V, Mincheva-Nilsson L
J Immunol, 2013;191(11):5515-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells.
Cancer Gene Ther., 2012;19(9):652-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
Authors: Neumann S, Bidon T, Branschadel M, Krippner-Heidenreich A, Scheurich P, Doszczak M
PLoS ONE, 2012;7(8):e42526.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dexamethasone counteracts the anti-osteoclastic, but not the anti-leukemic, activity of TNF-related apoptosis inducing ligand (TRAIL).
Authors: Zauli G, Rimondi E, Celeghini C, Milani D, Secchiero P
J. Cell. Physiol., 2010;222(2):357-64.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients.
Authors: Stary G, Klein I, Kohlhofer S, Koszik F, Scherzer T, Mullauer L, Quendler H, Kohrgruber N, Stingl G
Blood, 2009;114(18):3854-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A novel p53 rescue compound induces p53-dependent growth arrest and sensitises glioma cells to Apo2L/TRAIL-induced apoptosis.
Authors: Weinmann L, Wischhusen J, Demma MJ, Naumann U, Roth P, Dasmahapatra B, Weller M
Cell Death Differ., 2008;15(4):718-729.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Diallyl trisulfide increases the effectiveness of TRAIL and inhibits prostate cancer growth in an orthotopic model: molecular mechanisms.
Authors: Shankar S, Chen Q, Ganapathy S, Singh KP, Srivastava RK
Mol. Cancer Ther., 2008;7(8):2328-38.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
P53-mediated upregulation of DcR1 impairs oxaliplatin/TRAIL-induced synergistic anti-tumour potential in colon cancer cells.
Authors: Toscano F, Fajoui ZE, Gay F, Lalaoui N, Parmentier B, Chayvialle JA, Scoazec JY, Micheau O, Abello J, Saurin JC
Oncogene, 2008;27(30):4161-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
Authors: Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ
Exp. Hematol., 2008;36(12):1660-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential effects of chemotherapeutic drugs versus the MDM-2 antagonist nutlin-3 on cell cycle progression and induction of apoptosis in SKW6.4 lymphoblastoid B-cells.
Authors: Barbarotto E, Corallini F, Rimondi E, Fadda R, Mischiati C, Grill V, Vaccarezza M, Celeghini C
J. Cell. Biochem., 2008;104(2):595-605.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
J. Exp. Med., 2007;204(3):667-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
HDAC inhibitors enhance the apoptosis-inducing potential of TRAIL in breast carcinoma.
Authors: Singh TR, Shankar S, Srivastava RK
Oncogene, 2005;24(29):4609-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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