Human TRAIL/TNFSF10 Quantikine ELISA Kit

(20 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)
  • Sensitivity
    7.87 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human TRAIL
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC177, Quantikine Immunoassay Control Set 943 Human TRAIL/TNFSF10 - Please Inquire
Product Summary
The Quantikine Human TRAIL Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TRAIL in cell culture supernates, serum, plasma, cell lysates, and saliva. It contains NS0-expressed recombinant human TRAIL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TRAIL showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TRAIL

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean102203408102202407
Standard Deviation3.455.73237.5912.217.8
CV%3.42.85.67.56.14.4

Cell Culture Supernates, Cell Lysates, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean9519339196191388
Standard Deviation4.268.8124.38.314.822.2
CV%4.54.66.28.67.75.7

Recovery

The recovery of TRAIL spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 92-105
EDTA Plasma (n=4) 103 96-112
Heparin Plasma (n=4) 103 98-115
Serum (n=4) 96 91-102
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TRAIL were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TRAIL/TNFSF10 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TRAIL/TNFSF10
TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.
    • Long Name
      TNF-related Apoptosis-inducing Ligand
    • Entrez Gene IDs
      8743 (Human); 22035 (Mouse);
    • Alternate Names
      Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    20 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Expression of osteoprotegerin and its ligands, RANKL and TRAIL, in rheumatoid arthritis
      Sci Rep, 2016;6(0):29713.
      Species: Human
      Sample Type: Serum
    2. Clonal evolution in patients with chronic lymphocytic leukaemia developing resistance to BTK inhibition
      Nat Commun, 2016;7(0):11589.
      Species: Human
      Sample Type: Plasma
    3. TRAIL Is Decreased Before 20 Weeks Gestation in Women with Hypertensive Disorders of Pregnancy.
      Authors: Zhou C, Long Y, Yang H, Zhu C, Ma Q, Zhang Y
      PLoS ONE, 2015;10(6):e0128425.
      Species: Human
      Sample Type: Plasma
    4. Silencing Daxx increases the anti-tumor activity of a TRAIL/shRNA Bcl-xL-expressing oncolytic adenovirus through enhanced viral replication and cellular arrest.
      Authors: Kang S, Kang D, Bakhtiar Ul Islam S, Je S, Kim J, Song J
      Cell Signal, 2015;27(6):1214-24.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Clinically applicable human adipose tissue-derived mesenchymal stem cells delivering therapeutic genes to brainstem gliomas.
      Authors: Choi S, Lee Y, Kwak P, Lee J, Kim S, Lee S, Phi J, Wang K, Song J, Song S, Joo K, Kim S
      Cancer Gene Ther, 2015;22(6):302-11.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. TRAIL-receptor preferences in pancreatic cancer cells revisited: Both TRAIL-R1 and TRAIL-R2 have a licence to kill.
      Authors: Mohr A, Yu R, Zwacka R
      BMC Cancer, 2015;15(0):494.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib.
      Authors: Ray A, Tian Z, Das D, Coffman R, Richardson P, Chauhan D, Anderson K
      Leukemia, 2014;28(8):1716-24.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Systemic anticancer neural stem cells in combination with a cardiac glycoside for glioblastoma therapy.
      Authors: Teng J, Hejazi S, Badr C, Tannous B
      Stem Cells, 2014;32(8):2021-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
      Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
      J. Immunol., 2012;188(3):1002-10.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Potential role of soluble TRAIL in epithelial injury in children with severe RSV infection.
      Authors: Bem RA, Bos AP, Wosten-van Asperen RM, Bruijn M, Lutter R, Sprick MR, van Woensel JB
      Am. J. Respir. Cell Mol. Biol., 2010;42(6):697-705.
      Species: Human
      Sample Type: BALF
    11. Prognostic value of apoptosis markers in advanced heart failure patients.
      Authors: Niessner A, Hohensinner PJ, Rychli K, Neuhold S, Zorn G, Richter B, Hulsmann M, Berger R, Mortl D, Huber K, Wojta J, Pacher R
      Eur. Heart J., 2009;30(7):789-96.
      Species: Human
      Sample Type: Plasma
    12. Conjunctival sac fluid contains elevated levels of soluble TRAIL: implications for the anti-tumoral surveillance of the anterior surface of the eye.
      Authors: Secchiero P, Lamberti G, Corallini F, Melloni E, Guarnotta C, Sebastiani A, Zauli G
      J. Cell. Physiol., 2009;218(1):199-204.
      Species: Human
      Sample Type: Conjunctival Fluid
    13. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
      Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
      Stem Cells, 2008;26(11):2955-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
      Authors: Chamoux E, Houde N, L'Eriger K, Roux S
      J. Cell. Physiol., 2008;216(2):536-42.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Insulin down-regulates TRAIL expression in vascular smooth muscle cells both in vivo and in vitro.
      Authors: Corallini F, Celeghini C, Rizzardi C, Pandolfi A, Di Silvestre S, Vaccarezza M, Zauli G
      J. Cell. Physiol., 2007;212(1):89-95.
      Species: Human
      Sample Type: Serum
    16. Gene modulatory effects, pharmacokinetics, and clinical tolerance of interferon-alpha1b: a second member of the interferon-alpha family.
      Authors: Masci P, Olencki T, Wood L, Rybicki L, Jacobs B, Williams B, Faber P, Bukowski R, Tong K, Borden EC
      Clin. Pharmacol. Ther., 2007;81(3):354-61.
      Species: Human
      Sample Type: Serum
    17. TRAIL limits excessive host immune responses in bacterial meningitis.
      Authors: Hoffmann O, Priller J, Prozorovski T, Schulze-Topphoff U, Baeva N, Lunemann JD, Aktas O, Mahrhofer C, Stricker S, Zipp F, Weber JR
      J. Clin. Invest., 2007;117(7):2004-13.
      Species: Human
      Sample Type: CSF
    18. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
      Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
      Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
      Species: Human
      Sample Type: Plasma
    19. HIV-1 tat protein and cell proliferation and survival: a brief review.
      Authors: Gibellini D, Vitone F, Schiavone P, Re MC
      New Microbiol., 2005;28(2):95-109.
      Species: Human
      Sample Type: Plasma
    20. Evidence for a role of TNF-related apoptosis-inducing ligand (TRAIL) in the anemia of myelodysplastic syndromes.
      Authors: Campioni D, Secchiero P, Corallini F, Melloni E, Capitani S, Lanza F, Zauli G
      Am. J. Pathol., 2005;166(2):557-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 20 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 943 Human TRAIL/TNFSF10

    Ctrl QC177
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Cell Lysis Buffer 3 Concentrate (5X, 1 x 21 mL)

    ELISA 895366
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