Human TRAIL/TNFSF10 Quantikine ELISA Kit

  (21 citations)
(1 Review)
    
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Assay Procedure
Citations (21)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)
  • Sensitivity
    7.87 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human TRAIL
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC177 , Quantikine Immunoassay Control Set 943 Human TRAIL/TNFSF10 - Please Inquire
Product Summary
The Quantikine Human TRAIL Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TRAIL in cell culture supernates, serum, plasma, cell lysates, and saliva. It contains NS0-expressed recombinant human TRAIL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TRAIL showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TRAIL

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 95 193 391 96 191 388
Standard Deviation 4.26 8.81 24.3 8.3 14.8 22.2
CV% 4.5 4.6 6.2 8.6 7.7 5.7

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 102 203 408 102 202 407
Standard Deviation 3.45 5.73 23 7.59 12.2 17.8
CV% 3.4 2.8 5.6 7.5 6.1 4.4

Recovery

The recovery of TRAIL spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 92-105
EDTA Plasma (n=4) 103 96-112
Heparin Plasma (n=4) 103 98-115
Serum (n=4) 96 91-102
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TRAIL were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 TRAIL/TNFSF10 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TRAIL/TNFSF10
TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.
  • Long Name:
    TNF-related Apoptosis-inducing Ligand
  • Entrez Gene IDs:
    8743 (Human); 22035 (Mouse)
  • Alternate Names:
    Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Sample Type
  1. Anti-tumor effects of ONC201 in combination with VEGF-inhibitors significantly impacts colorectal cancer growth and survival in vivo through complementary non-overlapping mechanisms
    Authors: J Wagner, CL Kline, L Zhou, V Khazak, WS El-Deiry
    J. Exp. Clin. Cancer Res., 2018;37(1):11.
    Species: Mouse
    Sample Type: Serum
  2. Clonal evolution in patients with chronic lymphocytic leukaemia developing resistance to BTK inhibition
    Nat Commun, 2016;7(0):11589.
    Species: Human
    Sample Type: Plasma
  3. Expression of osteoprotegerin and its ligands, RANKL and TRAIL, in rheumatoid arthritis
    Sci Rep, 2016;6(0):29713.
    Species: Human
    Sample Type: Serum
  4. TRAIL Is Decreased Before 20 Weeks Gestation in Women with Hypertensive Disorders of Pregnancy.
    Authors: Zhou C, Long Y, Yang H, Zhu C, Ma Q, Zhang Y
    PLoS ONE, 2015;10(6):e0128425.
    Species: Human
    Sample Type: Plasma
  5. Silencing Daxx increases the anti-tumor activity of a TRAIL/shRNA Bcl-xL-expressing oncolytic adenovirus through enhanced viral replication and cellular arrest.
    Authors: Kang S, Kang D, Bakhtiar Ul Islam S, Je S, Kim J, Song J
    Cell Signal, 2015;27(6):1214-24.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Clinically applicable human adipose tissue-derived mesenchymal stem cells delivering therapeutic genes to brainstem gliomas.
    Authors: Choi S, Lee Y, Kwak P, Lee J, Kim S, Lee S, Phi J, Wang K, Song J, Song S, Joo K, Kim S
    Cancer Gene Ther, 2015;22(6):302-11.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. TRAIL-receptor preferences in pancreatic cancer cells revisited: Both TRAIL-R1 and TRAIL-R2 have a licence to kill.
    Authors: Mohr A, Yu R, Zwacka R
    BMC Cancer, 2015;15(0):494.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib.
    Authors: Ray A, Tian Z, Das D, Coffman R, Richardson P, Chauhan D, Anderson K
    Leukemia, 2014;28(8):1716-24.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Systemic anticancer neural stem cells in combination with a cardiac glycoside for glioblastoma therapy.
    Authors: Teng J, Hejazi S, Badr C, Tannous B
    Stem Cells, 2014;32(8):2021-32.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
    Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
    J. Immunol., 2012;188(3):1002-10.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Potential role of soluble TRAIL in epithelial injury in children with severe RSV infection.
    Authors: Bem RA, Bos AP, Wosten-van Asperen RM, Bruijn M, Lutter R, Sprick MR, van Woensel JB
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):697-705.
    Species: Human
    Sample Type: BALF
  12. Prognostic value of apoptosis markers in advanced heart failure patients.
    Authors: Niessner A, Hohensinner PJ, Rychli K, Neuhold S, Zorn G, Richter B, Hulsmann M, Berger R, Mortl D, Huber K, Wojta J, Pacher R
    Eur. Heart J., 2009;30(7):789-96.
    Species: Human
    Sample Type: Plasma
  13. Conjunctival sac fluid contains elevated levels of soluble TRAIL: implications for the anti-tumoral surveillance of the anterior surface of the eye.
    Authors: Secchiero P, Lamberti G, Corallini F, Melloni E, Guarnotta C, Sebastiani A, Zauli G
    J. Cell. Physiol., 2009;218(1):199-204.
    Species: Human
    Sample Type: Conjunctival Fluid
  14. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
    Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
    Stem Cells, 2008;26(11):2955-63.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
    Authors: Chamoux E, Houde N, L'Eriger K, Roux S
    J. Cell. Physiol., 2008;216(2):536-42.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Insulin down-regulates TRAIL expression in vascular smooth muscle cells both in vivo and in vitro.
    Authors: Corallini F, Celeghini C, Rizzardi C, Pandolfi A, Di Silvestre S, Vaccarezza M, Zauli G
    J. Cell. Physiol., 2007;212(1):89-95.
    Species: Human
    Sample Type: Serum
  17. Gene modulatory effects, pharmacokinetics, and clinical tolerance of interferon-alpha1b: a second member of the interferon-alpha family.
    Authors: Masci P, Olencki T, Wood L, Rybicki L, Jacobs B, Williams B, Faber P, Bukowski R, Tong K, Borden EC
    Clin. Pharmacol. Ther., 2007;81(3):354-61.
    Species: Human
    Sample Type: Serum
  18. TRAIL limits excessive host immune responses in bacterial meningitis.
    Authors: Hoffmann O, Priller J, Prozorovski T, Schulze-Topphoff U, Baeva N, Lunemann JD, Aktas O, Mahrhofer C, Stricker S, Zipp F, Weber JR
    J. Clin. Invest., 2007;117(7):2004-13.
    Species: Human
    Sample Type: CSF
  19. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
    Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
    Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
    Species: Human
    Sample Type: Plasma
  20. HIV-1 tat protein and cell proliferation and survival: a brief review.
    Authors: Gibellini D, Vitone F, Schiavone P, Re MC
    New Microbiol., 2005;28(2):95-109.
    Species: Human
    Sample Type: Plasma
  21. Evidence for a role of TNF-related apoptosis-inducing ligand (TRAIL) in the anemia of myelodysplastic syndromes.
    Authors: Campioni D, Secchiero P, Corallini F, Melloni E, Capitani S, Lanza F, Zauli G
    Am. J. Pathol., 2005;166(2):557-63.
    Species: Human
    Sample Type: Cell Culture Supernates
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Quantikine Immunoassay Control Set 943 Human TRAIL/TNFSF10

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Quantikine ELISA Wash Buffer 1

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Cell Lysis Buffer 3 Concentrate (5X, 1 x 21 mL)

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 Human TRAIL/TNFSF10 Quantikine ELISA Kit DTRL00
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