Home / TRAILR1/TNFRSF10A / Human TRAILR1/TNFRSF10A PE-conjugated Antibody

Human TRAILR1/TNFRSF10A PE-conjugated Antibody

Catalog #: FAB347P
31 Citations Datasheet / COA / SDS

Discontinued Product

FAB347P has been discontinued.
View all TRAILR1/TNFRSF10A products.
Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry.
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Citations (31)
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Human TRAILR1/TNFRSF10A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human TRAIL R1/TNFRSF10A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAIL R2, rhTRAIL R3, rhTRAIL R4, or rhDcR3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 69036
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRAIL R1/TNFRSF10A
Ala24-Asn239
Accession # AAC51226
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRAIL R1/TNFRSF10A antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TRAIL R1/TNFRSF10A PE-conjugated Monoclonal Antibody (Catalog # FAB347P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAILR1/TNFRSF10A

Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL RI is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References
  1. Pan, G. et al. (1997) Science 276:111.
  2. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 1
Entrez Gene IDs
8797 (Human)
Alternate Names
APO2; CD261 antigen; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4 TRAIL receptor 1; DR4; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL R1; TRAILR1; TRAIL-R1; TRAILR-1; TRAILR1MGC9365; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily, member 10a

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Citations for Human TRAILR1/TNFRSF10A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Accumulation of autophagosomes in breast cancer cells induces TRAIL resistance through downregulation of surface expression of death receptors 4 and 5.
    Authors: Di X, Zhang G, Zhang Y et al.
    Oncotarget
  2. H-Ras regulation of TRAIL death receptor mediated apoptosis.
    Authors: Chen Jun-Jie, Bozza William P, Di Xu et al.
    Oncotarget.
  3. Cytokeratin 8/18 protects breast cancer cell lines from TRAIL-induced apoptosis
    Authors: Bozza WP, Zhang Y, Zhang B.
    Oncotarget
  4. Induction of Breast Cancer Cell Apoptosis by TRAIL and Smac Mimetics: Involvement of RIP1 and cFLIP
    Authors: Christian Holmgren, Ellen Sunström Thörnberg, Victoria Granqvist, Christer Larsson
    Current Issues in Molecular Biology
  5. TRAIL sensitivity of nasopharyngeal cancer cells involves redox dependent upregulation of TMTC2 and its interaction with membrane caspase-3
    Authors: D Raman, P Tay, JL Hirpara, D Liu, S Pervaiz
    Redox Biology, 2021-11-20;48(0):102193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis
    Authors: Yu Ren, Xue Wang, Shuaishuai Huang, Yangkai Xu, Guobin Weng, Rui Yu
    Frontiers in Pharmacology
  7. Combination therapy with c-met inhibitor and TRAIL enhances apoptosis in dedifferentiated liposarcoma patient-derived cells
    Authors: EB Jo, YS Lee, H Lee, JB Park, H Park, YL Choi, D Hong, SJ Kim
    BMC Cancer, 2019-05-24;19(1):496.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. miR-494 Sensitizes Gastric Cancer Cells to TRAIL Treatment Through Downregulation of Survivin
    Authors: S Xu, D Li, T Li, L Qiao, K Li, L Guo, Y Liu
    Cell. Physiol. Biochem., 2018-12-07;51(5):2212-2223.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  9. Kaempferol Sensitizes Human Ovarian Cancer Cells-OVCAR-3 and SKOV-3 to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis via JNK/ERK-CHOP Pathway and Up-Regulation of Death Receptors 4 and 5
    Authors: Y Zhao, B Tian, Y Wang, H Ding
    Med. Sci. Monit., 2017-10-26;23(0):5096-5105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. TRAIL regulatory receptors constrain human hepatic stellate cell apoptosis
    Authors: HD Singh, I Otano, K Rombouts, KP Singh, D Peppa, US Gill, K Böttcher, PTF Kennedy, J Oben, M Pinzani, H Walczak, G Fusai, WMC Rosenberg, MK Maini
    Sci Rep, 2017-07-17;7(1):5514.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
    Authors: Warat M, Sadowski T, Szliszka E, Krol W, Czuba Z
    Molecules, 2015-01-08;20(1):900-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages.
    Authors: Warat M, Szliszka E, Korzonek-Szlacheta I, Krol W, Czuba Z
    Int J Mol Sci, 2014-06-27;15(7):11510-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells.
    Authors: Tang X, Lu J, Tu H, Huang K, Fu R, Cao G, Huang M, Cheng L, Dai L, Zhang L
    Anticancer Res, 2014-02-01;34(2):729-34.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
    Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo
    Stem Cells Transl Med, 2014-01-16;3(2):172-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway.
    Authors: Gupta, Subash C, Francis, Sajin K, Nair, Mangalam, Mo, Yin-Yuan, Aggarwal, Bharat B
    J Biol Chem, 2013-09-27;288(45):32343-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia.
    Authors: Gojo I, Tan M, Fang H, Sadowska M, Lapidus R, Baer M, Carrier F, Beumer J, Anyang B, Srivastava R, Espinoza-Delgado I, Ross D
    Clin Cancer Res, 2013-02-12;19(7):1838-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Smad7 protein induces interferon regulatory factor 1-dependent transcriptional activation of caspase 8 to restore tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis.
    Authors: Hong S, Kim H, Kim J, Ha H, Kim Y, Bae E, Kim T, Lee K, Kim S
    J Biol Chem, 2012-12-19;288(5):3560-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Mislocalization of death receptors correlates with cellular resistance to their cognate ligands in human breast cancer cells
    Authors: Jun-Jie Chen, H.-C. Jennifer Shen, Leslie A. Rivera Rosado, Yaqin Zhang, Xu Di, Baolin Zhang
    Oncotarget
  19. TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity.
    Authors: Kalb ML, Glaser A, Stary G, Koszik F, Stingl G
    J. Immunol., 2012-01-09;188(4):1583-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. WEE1 Inhibition Sensitizes Basal Breast Cancer Cells to TRAIL-Induced Apoptosis
    Authors: Sireesha V. Garimella, Andrea Rocca, Stanley Lipkowitz
    Molecular Cancer Research
  21. TNFR1 and TNFR2 regulate the extrinsic apoptotic pathway in myeloma cells by multiple mechanisms
    Authors: H Rauert, T Stühmer, R Bargou, H Wajant, D Siegmund
    Cell Death & Disease
  22. The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells.
    Authors: Szliszka E, Helewski KJ, Mizgala E, Krol W
    Int. J. Oncol., 2011-07-06;39(4):771-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response.
    Authors: Kang Z, Chen J, Yu Y, Li B, Sun S, Zhang B, Cao L
    Clin Cancer Res, 2011-03-08;17(10):3181-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Cisplatin sensitizes human hepatocellular carcinoma cells, but not hepatocytes and mesenchymal stem cells, to TRAIL within a therapeutic window partially depending on the upregulation of DR5
    Authors: Bo Zhang, Hong Shan, Dan Li, Zheng-Ran Li, Kang-Shun Zhu, Zai-Bo Jiang et al.
    Oncology Reports
  25. Epigenetic silencing of death receptor 4 mediates tumor necrosis factor-related apoptosis-inducing ligand resistance in gliomas.
    Authors: Elias A, Siegelin MD, Steinmuller A, von Deimling A, Lass U, Korn B, Mueller W
    Clin. Cancer Res., 2009-08-25;15(17):5457-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Gene therapy using TRAIL-secreting human umbilical cord blood-derived mesenchymal stem cells against intracranial glioma.
    Authors: Kim SM, Lim JY, Park SI, Jeong CH, Oh JH, Jeong M, Oh W, Park SH, Sung YC, Jeun SS
    Cancer Res., 2008-12-01;68(23):9614-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
    Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
    Stem Cells, 2008-09-04;26(11):2955-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. TRAIL recombinant adenovirus triggers robust apoptosis in multidrug-resistant HL-60/Vinc cells preferentially through death receptor DR5.
    Authors: Wu CH, Kao CH, Safa AR
    Hum. Gene Ther., 2008-07-01;19(7):731-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
    Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
    J. Exp. Med., 2007-03-12;204(3):667-80.
    Species: Human
    Sample Types: Serum
    Applications: Flow Cytometry
  30. A genome-wide loss-of-function screen identifies SLC26A2 as a novel mediator of TRAIL resistance
    Authors: Dimberg L, Towers CG, Behbakht K et al.
    Mol. Cancer Res
  31. Circulating Tumor Cells Develop Resistance to TRAIL-Induced Apoptosis Through Autophagic Removal of Death Receptor 5: Evidence from an In Vitro Model
    Authors: Twomey JD, Zhang B.
    Cancers (Basel)

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