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Human UbcH10/UBE2C Antibody

Catalog #: A-650
3 Citations Datasheet / COA / SDS

Discontinued Product

A-650 has been discontinued.
View all UbcH10/UBE2C products.
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UbcH10/UBE2C Antibody Summary

Species Reactivity
Human
Specificity
Detects human UbcH10/UBE2C in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Full-length, highly purified human UbcH10 protein
Formulation
Supplied in a solution of PBS and Glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Recommendeddilution range for Western blot analysis is 1:100 to 1:1000. Non-functional for immunoprecipitation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: UbcH10/UBE2C

UbcH10 is an essential mediator of mitotic destruction events and cell cycle progression.  It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex.  This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle.

Entrez Gene IDs
11065 (Human); 68612 (Mouse); 296368 (Rat)
Alternate Names
cyclin-selective ubiquitin carrier protein; dJ447F3.2; EC 6.3.2.19; UbcH10; UBCH10mitotic-specific ubiquitin-conjugating enzyme; UBE2C; Ubiquitin carrier protein C; ubiquitin carrier protein E2-C; ubiquitin-conjugating enzyme E2 C; ubiquitin-conjugating enzyme E2C; Ubiquitin-protein ligase C

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Citations for Human UbcH10/UBE2C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Growth inhibition associated with disruption of the actin cytoskeleton by Latrunculin A in rhabdomyosarcoma cells
    Authors: J Würtemberg, D Tchessalov, C Regina, C Bauer, M Schneider, AJ Wagers, S Hettmer
    PLoS ONE, 2020-09-08;15(9):e0238572.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression.
    Authors: Shen, Zhihua, Jiang, Xiaofan, Zeng, Chao, Zheng, Shaojian, Luo, Botao, Zeng, Yumei, Ding, Ranran, Jiang, Hanguo, He, Qiyi, Guo, Junli, Jie, Wei
    BMC Cancer, 2013-04-15;13(0):192.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  3. Skp1-Cul1-F-box ubiquitin ligase (SCF(betaTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry.
    Authors: Burrows A, Prokop J, Summers M
    J Biol Chem, 2012-10-01;287(46):39021-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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