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Human UbcH7/UBE2L3 Antibody

Catalog #: A-640
3 Citations Datasheet / COA / SDS

Discontinued Product

A-640 has been discontinued.
View all UbcH7/UBE2L3 products.
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UbcH7/UBE2L3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human UbcH7/UBE2L3 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Full-length, highly purified human UbcH7 protein
Formulation
Supplied in a solution of PBS and Glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Recommended dilution range for Western blot analysis is 1:100 to 1:1000.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: UbcH7/UBE2L3

UbcH7 mediates the selective degradation of short-lived and abnormal proteins and is highly homologous to UbcH5.  UbcH7 interacts with the HECT domain of E6-AP and the RING domain of c-Cbl, and can mediate the multi-ubiquitination of many different types of protein substrates. 

Entrez Gene IDs
7332 (Human); 22195 (Mouse); 363836 (Rat)
Alternate Names
E2-F1; EC 6.3.2.19; L-UBC; UBCE7; UbcH7; UBCH7UbcM4; UbcM4; UBE2L3; Ubiquitin carrier protein L3; ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; Ubiquitin-protein ligase L3

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Citations for Human UbcH7/UBE2L3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The ubiquitin-conjugating enzymes UBE2N, UBE2L3 and UBE2D2/3 are essential for Parkin-dependent mitophagy.
    Authors: Geisler S, Vollmer S, Golombek S, Kahle P
    J Cell Sci, 2014-06-06;127(0):3280-93.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  2. Proteasome proteolysis supports stimulated platelet function and thrombosis.
    Authors: Gupta N, Li W, Willard B, Silverstein R, McIntyre T
    Arterioscler Thromb Vasc Biol, 2013-10-31;34(1):160-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity.
    Authors: Maezawa S, Fukushima R, Matsushita T
    PLoS ONE, 2012-07-11;7(7):e39511.
    Species: Human
    Sample Types: Whole Cells

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