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Human UBE2M/Ubc12 Antibody

Catalog #: A-655
2 Citations Datasheet / COA / SDS

Discontinued Product

A-655 has been discontinued.
View all UBE2M/Ubc12 products.
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UBE2M/Ubc12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human UBE2M/Ubc12 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Full-length, highly purified human UbcH12 protein
Formulation
Supplied in a solution of PBS and Glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Recommended dilution range for Western blot analysis is 1:100 to 1:1000.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: UBE2M/Ubc12

NEDDylation requires the coordinated activities of UbcH12 and the APP-BPI/Uba3 activating enzyme.  This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based families of ubiquitin protein ligases).  The NEDD8 pathway is essential for cell viability in fission yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.

Entrez Gene IDs
9040 (Human); 22192 (Mouse); 361509 (Rat)
Alternate Names
EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; UBC12; UBC12NEDD8-conjugating enzyme Ubc12; UbcH12; UBC-RS2; UBE2M; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog

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Citations for Human UBE2M/Ubc12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. RNF111-dependent neddylation activates DNA damage-induced ubiquitination.
    Authors: Ma T, Chen Y, Zhang F, Yang C, Wang S, Yu X
    Mol Cell, 2013-02-07;49(5):897-907.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. A metal-based inhibitor of NEDD8-activating enzyme.
    Authors: Zhong H, Yang H, Chan D, Leung C, Wang H, Ma D
    PLoS ONE, 2012-11-19;7(11):e49574.
    Applications: Western Blot

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