Human ULBP-2/5/6 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry.
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Product Details
Citations (21)
Supplemental Products
Reviews (1)

Human ULBP-2/5/6 APC-conjugated Antibody Summary

Species Reactivity
Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
Monoclonal Mouse IgG2A Clone # 165903
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with human ULBP-2
Accession # Q9BZM5
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ULBP-2/5/6 antibody in HT1080 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 APC-conjugated Monoclonal Antibody (Catalog # FAB1298A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human ULBP-2/5/6 by Flow Cytometry View Larger

Detection of Human ULBP-2/5/6 by Flow Cytometry Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-2/5/6

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002)
Long Name
UL16 Binding Protein-2/5/6
Alternate Names
ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; UL16 binding protein 2; ULBP2; ULBP-2/5/6

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Citations for Human ULBP-2/5/6 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Fumarate Upregulates Surface Expression of ULBP2/ULBP5 by Scavenging Glutathione Antioxidant Capacity
    Authors: RI Høgh, A Droujinine, SH Møller, SD Jepsen, M Mellergaar, L Andresen, S Skov
    J. Immunol., 2020-03-06;0(0):.
  2. Development of allogeneic HSC-engineered iNKT cells for off-the-shelf cancer immunotherapy
    Authors: Yan-Ruide Li, Yang Zhou, Yu Jeong Kim, Yanni Zhu, Feiyang Ma, Jiaji Yu et al.
    Cell Reports Medicine
  3. Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding
    Authors: R Fu, F Yu, W Wu, J Liu, J Li, F Guo, L Xu, F Wang, X Cui
    International immunopharmacology, 2021-10-19;101(0):108195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells
    Authors: X Zhong, H Liao, S Hu, K Luo, H Zhu
    Molecular and cellular probes, 2021-07-12;59(0):101759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity
    Authors: D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto
    Stem Cell Reports, 2019-12-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. EGFR-Specific Tyrosine Kinase Inhibitor Modifies NK Cell-Mediated Antitumoral Activity against Ovarian Cancer Cells
    Authors: N Mallmann-G, Y Sax, R Kimmig, S Lang, S Brandau
    Int J Mol Sci, 2019-09-22;20(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy
    Authors: M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery
    Cell Death Dis, 2019-09-20;10(10):695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor Cells
    Authors: Jaydeep Bhat, Samuel Dubin, Alexandra Dananberg, Elgar Susanne Quabius, Juergen Fritsch, C. Marie Dowds et al.
    Frontiers in Immunology
  9. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function
    Authors: A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard
    BMC Immunol., 2019-01-29;20(1):8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Overcoming Target Driven Fratricide for T Cell Therapy
    Authors: Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al.
    Frontiers in Immunology
  11. Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML
    Authors: AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg
    Clin. Cancer Res., 2018-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy
    Authors: Ryoji Kamei, Kiyoshi Yoshimura, Shigefumi Yoshino, Moeko Inoue, Tetsuhiko Asao, Masanori Fuse et al.
    Oncology Letters
  13. Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4? T Cells
    Authors: A Tremblay-M, J Bruneau, B Lebouché, I Lisovsky, R Song, NF Bernard
    Viruses, 2017-10-12;9(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. T Cells Infiltrating Diseased Liver Express Ligands for the NKG2D Stress Surveillance System
    Authors: Wei-Chen Huang
    J. Immunol, 2016-12-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
    Authors: Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W
    J Immunol, 2015-02-16;194(6):2930-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
    Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
    J Immunol, 2014-07-14;193(4):1654-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
    Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
    J Exp Med, 2013-11-04;210(12):2675-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Sensitizing primary acute lymphoblastic leukemia to natural killer cell recognition by induction of NKG2D ligands
    Authors: Laura Jardine, Sophie Hambleton, Venetia Bigley, Sarah Pagan, Xiao-Nong Wang, Matthew Collin
    Leukemia & Lymphoma
  19. Treatment of a Solid Tumor Using Engineered Drug-Resistant Immunocompetent Cells and Cytotoxic Chemotherapy
    Authors: Anindya Dasgupta, Jordan E. Shields, H. Trent Spencer
    Human Gene Therapy
  20. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012-06-06;86(16):8693-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression.
    Authors: Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S
    PLoS ONE, 2011-08-09;6(8):e23023.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human ULBP-2/5/6 APC-conjugated Antibody
By Stephane Hua on 07/05/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human