Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
Monoclonal Mouse IgG2A Clone # 165903
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with human ULBP-2 Accession # Q9BZM5
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry.
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PE‑conjugated Monoclonal Antibody (Catalog # FAB1298P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
multiple signaling pathways in primary NK cells, resulting in the production of
cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium
mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal
transduction pathway. The name ULBP derives from the original identification of
three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus
glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes
for ULBPs reside in a cluster of ten related genes, six of which encode
potentially functional glycoproteins. ULBP-2 has also been described under the
names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5
also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are
distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2
Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin.
Some family members, including ULBP-2, are anchored to the membrane via a
GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D
results in the activation of cytolytic activity and/or cytokine production by
these effector cells. The ULBPs are expressed on some tumor cells and have been
implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and
95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
Cosman, D. et al. (2001) Immunity 14:123.
Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
Sutherland, C. et al. (2002) J. Immunol. 168:671.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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