Human ULBP-2/5/6 PE-conjugated Antibody

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Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry.
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Citations (32)
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Human ULBP-2/5/6 PE-conjugated Antibody Summary

Species Reactivity
Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
Monoclonal Mouse IgG2A Clone # 165903
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with human ULBP-2
Accession # Q9BZM5
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ULBP-2/5/6 antibody in HT1080 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PE-conjugated Monoclonal Antibody (Catalog # FAB1298P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-2/5/6

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002)
Long Name
UL16 Binding Protein-2/5/6
Alternate Names
ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; UL16 binding protein 2; ULBP2; ULBP-2/5/6

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Citations for Human ULBP-2/5/6 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
    Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance
    Authors: Dario Gerace, Quan Zhou, Jennifer Hyoje-Ryu Kenty, Adrian Veres, Elad Sintov, Xi Wang et al.
    Cell Reports Medicine
  3. gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects
    Authors: NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest
    Nature, 2023-01-11;613(7945):743-750.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma
    Authors: Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai
    Translational Oncology, 2022-08-20;25(0):101521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity
    Authors: D Bernareggi, Q Xie, BC Prager, J Yun, LS Cruz, TV Pham, W Kim, X Lee, M Coffey, C Zalfa, P Azmoon, H Zhu, P Tamayo, JN Rich, DS Kaufman
    Nature Communications, 2022-04-07;13(1):1899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
    Authors: T Seki, Y Shimizu, K Ishii, Y Takahama, K Kato, T Yano
    Biomolecules, 2021-11-16;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CD34(+) progenitor-derived NK cell and gemcitabine combination therapy increases killing of ovarian cancer cells in NOD/SCID/IL2Rg(null) mice
    Authors: Van der Meer JMR, de Jonge PKJD, van der Waart AB et al.
  9. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis
    Authors: Y Zhu, J Xie, J Shi
    Bmc Biology, 2021-07-30;19(1):140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Expanded human NK cells armed with CAR uncouple potent anti-tumor activity from off-tumor toxicity against solid tumors
    Authors: Ana L. Portillo, Richard Hogg, Sophie M. Poznanski, Eduardo A. Rojas, Niamh J. Cashell, Joanne A. Hammill et al.
  11. Selection of tumor‑resistant variants following sustained natural killer cell‑mediated immune stress
    Authors: Thibault Carré, Jerome Thiery, Bassam Janji, Stéphane Terry, Gwendoline Gros, Guillaume Meurice et al.
    Oncology Reports
  12. Umbilical Cord Blood and iPSC-Derived Natural Killer Cells Demonstrate Key Differences in Cytotoxic Activity and KIR Profiles
    Authors: Benjamin H. Goldenson, Huang Zhu, YunZu Michele Wang, Naveen Heragu, Davide Bernareggi, Alessa Ruiz-Cisneros et al.
    Frontiers in Immunology
  13. The Oncoprotein SKI Acts as A Suppressor of NK Cell-Mediated Immunosurveillance in PDAC
    Authors: V Ponath, M Frech, M Bittermann, R Al Khayer, A Neubauer, C Brendel, E Pogge von
    Cancers (Basel), 2020-10-03;12(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells
    Authors: Sofie H. Møller, Maiken Mellergaard, Mikkel Madsen, Amaia V. Bermejo, Stine D. Jepsen, Marie H. Hansen et al.
    Frontiers in Immunology
  15. Genetic and Molecular Basis of Heterogeneous NK Cell Responses against Acute Leukemia
    Authors: DR Makanga, F Da Rin de, G David, C Willem, L Dubreuil, N Legrand, T Guillaume, P Peterlin, A Lebourgeoi, MC Béné, A Garnier, P Chevallier, K Gendzekhad, A Cesbron, K Gagne, B Clemenceau, C Retière
    Cancers (Basel), 2020-07-16;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Enhanced activation of human NK cells by drug-exposed hepatocytes
    Authors: Frank Fasbender, Martin Obholzer, Sarah Metzler, Regina Stöber, Jan G. Hengstler, Carsten Watzl
    Archives of Toxicology
  17. Potentiating Oncolytic Virus-Induced Immune-Mediated Tumor Cell Killing Using Histone Deacetylase Inhibition
    Authors: VA Jennings, GB Scott, AMS Rose, KJ Scott, G Migneco, B Keller, K Reilly, O Donnelly, H Peach, D Dewar, KJ Harrington, H Pandha, A Samson, RG Vile, AA Melcher, F Errington-
    Mol. Ther., 2019-04-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. PD-L1 upregulation in myeloma cells by panobinostat in combination with interferon-gamma
    Authors: Masami Iwasa, Takeshi Harada, Asuka Oda, Ariunzaya Bat-Erdene, Jumpei Teramachi, Hirofumi Tenshin et al.
  19. Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells
    Authors: Y Han, W Xie, DG Song, DJ Powell
    J Hematol Oncol, 2018-07-06;11(1):92.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  20. Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
    Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
    Oncotarget, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand
    PLoS Pathog, 2016-12-01;12(12):e1006015.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein
    Authors: Xiansheng Lin, Mei Huang, Fang Xie, Hangcheng Zhou, Ji Yang, Qiang Huang
  23. Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts
    Blood, 2016-03-24;127(23):2879-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Natural killer (NK) cells inhibit systemic metastasis of glioblastoma cells and have therapeutic effects against glioblastomas in the brain.
    Authors: Lee S, Kang W, Yoon Y, Jin J, Song H, Her J, Kang S, Hwang Y, Kang K, Joo K, Nam D
    BMC Cancer, 2015-12-24;15(1):1011.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.
    Authors: Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F
    Leukemia, 2015-03-27;29(9):1799-810.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Mutual Interaction between Human Multipotent Adult Progenitor Cells and NK Cells
    Authors: Sandra A. Jacobs, Jeroen Plessers, Jef Pinxteren, Valerie D. Roobrouck, Catherine M. Verfaillie, Stefaan W. Van Gool
    Cell Transplantation
  27. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
    Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
    J Immunol, 2014-07-14;193(4):1654-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Natural killer cell-mediated shedding of ULBP2.
    Authors: Wang, Ruipeng, Sun, Peter D
    PLoS ONE, 2014-03-10;9(3):e91133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  29. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman NC, Stutzman N, Zou Z, Sun PD
    J Leukoc Biol, 2011-11-01;91(2):299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression.
    Authors: Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S
    PLoS ONE, 2011-08-09;6(8):e23023.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011-02-25;6(2):e16899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Cytotoxicity of activated natural killer cells against pediatric solid tumors.
    Authors: Cho D, Shook DR, Shimasaki N
    Clin. Cancer Res., 2010-06-11;16(15):3901-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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