|Detection of Human USAG1 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7034) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USAG1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
USAG1 (also Ectodin and WISE; Wnt modulator in surface ectoderm) is a secreted, monomeric 26-32 kDa member of the sclerostin family of proteins. It exhibits restricted expression, being found in renal distal tubule cells (including macula densa), ameloblasts of teeth (for enamel formation), and cells of the dermal papilla. USAG1 binds BMP-2,-4,-6,and -7 with high affinity. This sequesters BMPs, making them unavailable for BMP receptor binding. It also binds LRP6, which, while weakly activating the LRP6 pathway, simultaneously blocks Wnt binding with strong Wnt-mediated signaling. Mature human USAG1 is 183 amino acids (aa) in length. It contains one CTCK domain (aa 75-170) plus two N-linked glycosylation sites. Mature human USAG1 (aa 24-206) shares 97% aa identity with mature mouse USAG1.
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