Human USAG1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human USAG1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human USAG1
    Phe24-Ser206
    Accession # Q6X4U4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human USAG1 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7034) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USAG1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: USAG1

USAG1 (also Ectodin and WISE; Wnt modulator in surface ectoderm) is a secreted, monomeric 26-32 kDa member of the sclerostin family of proteins. It exhibits restricted expression, being found in renal distal tubule cells (including macula densa), ameloblasts of teeth (for enamel formation), and cells of the dermal papilla. USAG1 binds BMP-2,-4,-6,and -7 with high affinity. This sequesters BMPs, making them unavailable for BMP receptor binding. It also binds LRP6, which, while weakly activating the LRP6 pathway, simultaneously blocks Wnt binding with strong Wnt-mediated signaling. Mature human USAG1 is 183 amino acids (aa) in length. It contains one CTCK domain (aa 75-170) plus two N-linked glycosylation sites. Mature human USAG1 (aa 24-206) shares 97% aa identity with mature mouse USAG1.

  • Long Name:
    Sclerostin Domain Containing Protein 1/Uterine Sensitization-associated Gene 1 Protein
  • Entrez Gene IDs:
    25928 (Human); 66042 (Mouse); 266803 (Rat)
  • Alternate Names:
    CDA019; cystine-knot containing secreted protein; DKFZp564D206; Ectodermal BMP inhibitor; Ectodin; sclerostin domain containing 1; sclerostin domain-containing protein 1; SOSTDC1; USAG1; USAG-1; USAG1uterine sensitization-associated protein-1; Uterine sensitization-associated gene 1 protein; WISE
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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