Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all USAG1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human USAG1
Phe24-Ser206
Accession # Q6X4U4

Specificity

Detects human USAG1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human USAG1 Antibody

Detection of Human USAG1 antibody by Western Blot.

Detection of Human USAG1 by Western Blot.

Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7034) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USAG1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human USAG1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human kidney (medulla) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USAG1

USAG1 (also Ectodin and WISE; Wnt modulator in surface ectoderm) is a secreted, monomeric 26-32 kDa member of the sclerostin family of proteins. It exhibits restricted expression, being found in renal distal tubule cells (including macula densa), ameloblasts of teeth (for enamel formation), and cells of the dermal papilla. USAG1 binds BMP-2,-4,-6,and -7 with high affinity. This sequesters BMPs, making them unavailable for BMP receptor binding. It also binds LRP6, which, while weakly activating the LRP6 pathway, simultaneously blocks Wnt binding with strong Wnt-mediated signaling. Mature human USAG1 is 183 amino acids (aa) in length. It contains one CTCK domain (aa 75-170) plus two N-linked glycosylation sites. Mature human USAG1 (aa 24-206) shares 97% aa identity with mature mouse USAG1.

Long Name

Sclerostin Domain Containing Protein 1/Uterine Sensitization-associated Gene 1 Protein

Alternate Names

CDA019, Ectodin, SOSTDC1, WISE

Entrez Gene IDs

25928 (Human); 66042 (Mouse); 266803 (Rat)

Gene Symbol

SOSTDC1

UniProt

Q6X4U4

Additional USAG1 Products

Product Documents for Human USAG1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human USAG1 Antibody

For research use only

Citations for Human USAG1 Antibody

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